Document Detail


Cytogenetic map for Anopheles nili: application for population genetics and comparative physical mapping.
MedLine Citation:
PMID:  20603229     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Anopheles nili is one of the major malaria vectors in Africa with a wide geographic distribution. However, the taxonomic and population genetic studies on this species are scarce. New research tools are urgently needed to genetically characterize this important malaria vector. In this study, a high-resolution cytogenetic map was developed for An. nili polytene chromosomes. Chromosomes were straightened and subdivided into 46 numbered divisions according to the banding pattern. Population analysis of An. nili females collected in Burkina Faso revealed the presence of two highly polymorphic inversions on the 2R chromosomal arm. A statistically significant departure from Hardy-Weinberg equilibrium due to a deficit in heterozygotes was detected for inversion 2Rb. To determine chromosome homologies and gene order conservation between An. nili and other major malaria vectors, PCR probes based on the An. gambiae coding sequences were mapped to An. nili chromosomes. Comparative mapping demonstrated that An. nili chromosomes have an An. stephensi-like arm association and that whole-arm translocations and paracentric inversions were the major types of rearrangement in evolution of these mosquitoes. The minimum number of fixed inversions among An. nili, An. gambiae, and An. stephensi was calculated using the Multiple Genome Rearrangements (MGR), Genome Rearrangements In Man and Mouse (GRIMM), and Sorting Permutation by Reversals and block-INterchanGes (SPRING) programs. The data suggest that the An. nili is, at least, as diverged from An. gambiae as An. stephensi. We provide evidence that 2La/a arrangement of An. gambiae is present in outgroup species An. nili and An. stephensi confirming the ancestral status of the 2La inversion in the An. gambiae complex. Availability of the new polytene chromosome map, polymorphic inversions, and physically mapped DNA markers for An. nili will further stimulate population genetic, taxonomic, and genomic studies of this neglected malaria vector.
Authors:
Maria V Sharakhova; Christophe Antonio-Nkondjio; Ai Xia; Cyrille Ndo; Parfait Awono-Ambene; Frederic Simard; Igor V Sharakhov
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-07-21
Journal Detail:
Title:  Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases     Volume:  11     ISSN:  1567-7257     ISO Abbreviation:  Infect. Genet. Evol.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-30     Completed Date:  2011-09-22     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  101084138     Medline TA:  Infect Genet Evol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  746-54     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anopheles / genetics*
Chromosome Inversion
Chromosome Mapping*
Cytogenetics
Female
Gene Order
Genetic Markers
Genetics, Population*
Humans
Polymorphism, Genetic
Polytene Chromosomes / ultrastructure
Sequence Homology
Grant Support
ID/Acronym/Agency:
5R21AI074729-02/AI/NIAID NIH HHS; 5R21AI079350-02/AI/NIAID NIH HHS; 5R21AI081023-02/AI/NIAID NIH HHS; R21 AI074729/AI/NIAID NIH HHS; R21 AI074729-01A1/AI/NIAID NIH HHS; R21 AI079350/AI/NIAID NIH HHS; R21 AI079350-02/AI/NIAID NIH HHS; R21 AI081023/AI/NIAID NIH HHS; R21 AI081023-02/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Genetic Markers
Comments/Corrections

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