Document Detail

Cytogenetic instability with balanced chromosome changes in an SV40 transformed human uroepithelial cell line.
MedLine Citation:
PMID:  2835156     Owner:  NLM     Status:  MEDLINE    
Cytogenetic analysis at the 15th, 34th, 50th, and 56th passages of an SV40 immortalized human uroepithelial cell line (SV-HUC-1) showed continuous chromosome change and marker formation. Throughout these passages the transformed cells maintained their epithelial morphology, were SV40 T antigen positive, did not shed infectious SV40 virus, and were repeatedly found to be nontumorigenic when innoculated into athymic nude mice. Each of the passages studied was characterized by extensive karyotypic changes due to formation, rearrangement, and disappearance of different markers. A marker involving chromosome 1 was stable at three of the passages studied, whereas markers involving the X chromosome changed at each passage studied. Because of the incorporation of several chromosomes or chromosome arms into markers, the karyotype was genetically balanced in the first passage studied, with no net loss or gain of chromosomal material despite a modal number of 44. In subsequent passages, despite continued instability and generation of new markers, there was a slight but additive loss of genomic balance which increased with time in culture. Since continued karyotypic rearrangements did not lead to tumorigenic conversion, it is probable that genetic instability coupled with selection for the most balanced genome may be important for the immortalization of this cell line.
L F Meisner; S Q Wu; B J Christian; C A Reznikoff
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer research     Volume:  48     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1988-06-15     Completed Date:  1988-06-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3215-20     Citation Subset:  IM    
State Laboratory of Hygiene, University of Wisconsin Health Sciences Center, Madison 53706.
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MeSH Terms
Cell Line, Transformed
Cell Transformation, Neoplastic*
Chromosome Aberrations*
Chromosome Disorders*
Chromosomes, Human*
Simian virus 40 / genetics*
Urinary Bladder

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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