| Cytogenetic evaluation and the association with polymorphisms of the CPY1A1 and NR1I3 genes in individuals exposed to BTEX. | |
| | |
MedLine Citation:
|
PMID: 23138419 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The gas station attendants are exposed daily to chemical agents that compose gasoline, such as BTEX (benzene, toluene, ethylbenzene, and xylene), and the exposure to these agents can cause a variety of effects on the human health. Among the various possible cell alterations associated with these exposures are the formation of micronuclei and of binucleated cells which are used as indicators of clastogenic action. Benzene, the main carcinogenic agent, is metabolized to more soluble forms and easily excreted by isoenzymes of cytochrome P450, such as CYP1A1. The CYP1A1 gene is highly polymorphic and one of its allele variations can be detected by the use of restriction endonucleasis MspI and is originated by the transition of a thymine by a cytosine (3798T>C), resulting in the polymorphic allele CYP1A1*2A. The objective of this study was to evaluate the cytogenetic damage induced by the exposure to BTEX and to associate it with the polymorphisms of the CYP1A1 and NR1I3 genes. Samples of exfoliated cells from the oral mucosa of 27 gas station attendants and from a control group were collected. The results found show that the group exposed to BTEX presents significantly higher alterations than those in the control group for micronuclei (MN; 6.85 ± 1.33 vs. 2.96 ± 1.91, P < 0.001) and for the total of nuclear alterations observed (MN + binucleated cells (BNC); 9.59 ± 4.73 vs. 5.07 ± 2.21, P < 0.001). When comparing the cytological alterations and the genotypes among the exposed individuals for the polymorphism 3798T>C of the CYP1A1 gene, homozygotes TT present MN + BNC significantly higher than carriers of the allele C (10.88 ± 5.36 vs. 5.33 ± 2.52, P = 0.028). No association was observed in the control group or for the NR1I3 gene. These results show that molecular and cytogenetic data can be used in the future as tools to monitor individuals exposed to such compounds. |
| | |
Authors:
|
João Carlos Fraga da Rosa; Marilu Fiegenbaum; Ane Lise Soledar; Matheus Souza Claus; Antonio Daniel de Souza Nunes; Valesca Veiga Cardoso |
Related Documents
:
|
22957059 - Genome-wide association study identified cnp12587 region underlying height variation in... 20662919 - Crispld2 polymorphisms are associated with non-syndromic cleft lip with or without clef... 23300579 - Partial deletion of the sulfate transporter slc13a1 is associated with an osteochondrod... 18640039 - Walker-warburg syndrome with pomt1 mutations can be associated with cleft lip and cleft... 23418889 - The genomic signature of trait-associated variants. 23215759 - Drd2 c313t and drd4 48-bp vntr polymorphisms and physical activity of healthy men in lo... 17073189 - Communication in genetic counselling for breast/ovarian cancer. 22579279 - Sibling rivalry among paralogs promotes evolution of the human brain. 8892669 - Microsatellite instability is an early genetic event in myelodysplastic syndrome but is... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-11-10 |
Journal Detail:
|
Title: Environmental monitoring and assessment Volume: - ISSN: 1573-2959 ISO Abbreviation: Environ Monit Assess Publication Date: 2012 Nov |
Date Detail:
|
Created Date: 2012-11-9 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8508350 Medline TA: Environ Monit Assess Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Laboratório de Mutagênese e Toxicologia, Centro Universitário Metodista-IPA, Rua Demetrio Ribeiro, 151 Apt 702, Porto Alegre, Rio Grande do Sul, Brazil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Reference values for heavy metals in soils of the Brazilian agricultural frontier in Southwestern Am...
Next Document: Accuracy of emergency physician-performed ultrasonography in the diagnosis of deep-vein thrombosis. ...