Document Detail


Cytogenetic evaluation and the association with polymorphisms of the CPY1A1 and NR1I3 genes in individuals exposed to BTEX.
MedLine Citation:
PMID:  23138419     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The gas station attendants are exposed daily to chemical agents that compose gasoline, such as BTEX (benzene, toluene, ethylbenzene, and xylene), and the exposure to these agents can cause a variety of effects on the human health. Among the various possible cell alterations associated with these exposures are the formation of micronuclei and of binucleated cells which are used as indicators of clastogenic action. Benzene, the main carcinogenic agent, is metabolized to more soluble forms and easily excreted by isoenzymes of cytochrome P450, such as CYP1A1. The CYP1A1 gene is highly polymorphic and one of its allele variations can be detected by the use of restriction endonucleasis MspI and is originated by the transition of a thymine by a cytosine (3798T>C), resulting in the polymorphic allele CYP1A1*2A. The objective of this study was to evaluate the cytogenetic damage induced by the exposure to BTEX and to associate it with the polymorphisms of the CYP1A1 and NR1I3 genes. Samples of exfoliated cells from the oral mucosa of 27 gas station attendants and from a control group were collected. The results found show that the group exposed to BTEX presents significantly higher alterations than those in the control group for micronuclei (MN; 6.85 ± 1.33 vs. 2.96 ± 1.91, P < 0.001) and for the total of nuclear alterations observed (MN + binucleated cells (BNC); 9.59 ± 4.73 vs. 5.07 ± 2.21, P < 0.001). When comparing the cytological alterations and the genotypes among the exposed individuals for the polymorphism 3798T>C of the CYP1A1 gene, homozygotes TT present MN + BNC significantly higher than carriers of the allele C (10.88 ± 5.36 vs. 5.33 ± 2.52, P = 0.028). No association was observed in the control group or for the NR1I3 gene. These results show that molecular and cytogenetic data can be used in the future as tools to monitor individuals exposed to such compounds.
Authors:
João Carlos Fraga da Rosa; Marilu Fiegenbaum; Ane Lise Soledar; Matheus Souza Claus; Antonio Daniel de Souza Nunes; Valesca Veiga Cardoso
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Publication Detail:
Type:  Journal Article     Date:  2012-11-10
Journal Detail:
Title:  Environmental monitoring and assessment     Volume:  185     ISSN:  1573-2959     ISO Abbreviation:  Environ Monit Assess     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-05-30     Completed Date:  2013-09-05     Revised Date:  2013-10-23    
Medline Journal Info:
Nlm Unique ID:  8508350     Medline TA:  Environ Monit Assess     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  5883-90     Citation Subset:  IM    
Affiliation:
Laboratório de Mutagênese e Toxicologia, Centro Universitário Metodista-IPA, Rua Demetrio Ribeiro, 151 Apt 702, Porto Alegre, Rio Grande do Sul, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Adult
Air Pollutants, Occupational / toxicity*
Benzene / toxicity
Benzene Derivatives / toxicity*
Cytochrome P-450 CYP1A1 / genetics*,  metabolism
Female
Humans
Male
Mutagens / toxicity*
Occupational Exposure / analysis*
Polymorphism, Single Nucleotide
Receptors, Cytoplasmic and Nuclear / genetics*,  metabolism
Toluene / toxicity
Xylenes / toxicity
Young Adult
Chemical
Reg. No./Substance:
0/Air Pollutants, Occupational; 0/Benzene Derivatives; 0/Mutagens; 0/Receptors, Cytoplasmic and Nuclear; 0/Xylenes; 0/constitutive androstane receptor; 108-88-3/Toluene; 71-43-2/Benzene; EC 1.14.14.1/Cytochrome P-450 CYP1A1; L5I45M5G0O/ethylbenzene

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