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Cytogenetic characterization reveals differences in nuclear organization of cystic cells among Brazilian species of Triatoma (Heteroptera, Reduviidae).
MedLine Citation:
PMID:  23035944     Owner:  NLM     Status:  Publisher    
Previous cytogenetic studies in triatomines have described the occurrence of holokinetic chromosomes, heterochromatin distribution and the location of rDNA sites, but few aspects of nuclear organization in this group have been discussed. In this study, we focused on ultrastructural and cytogenetic features and differences in cystic cells of seminiferous tubules between five species of Triatoma. We noted that cystic cells show evidence of polyploidy events and that heterochromatic blocks appear predominantly in the central region of the nuclei. Cytogenetic analyses have shown that there is variation in chromocenter number between species and that the central regions are AT-rich (DAPI+) and the periphery CG-rich (CMA+). Another characteristic is the distribution of 45S rDNA, which differs according to the chromosomal location of this sequence. We compared aspects of nuclear organization, polyploidy, heterochromatin, rDNA sites distribution, and methylation levels, as well as the relationships between five species of Triatoma from a cystic cell perspective.
Vanessa Bellini Bardella; Rosana Silistino de Souza; Juca Abramo Barrera San Martin; Célia Guadalupe Tardeli de Jesus Andrade; André Luís Laforga Vanzela; Maria Tercília Vilela de Azeredo-Oliveira
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-5
Journal Detail:
Title:  Cell biology international     Volume:  -     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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