Document Detail


Cytogenetic assays for mitotic poisons: the diploid Chinese hamster cell system.
MedLine Citation:
PMID:  6870196     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
When the diploid Chinese hamster cells (line Don) were treated with Colcemid (final concentrations 0.05 micrograms/ml and 2 mg/ml) for three hours and the mitotic arrest was reversed by washing and reincubation in the control medium, the cells were able to reorganize spindle and divide within 2-4 hr. When the cells were treated with vinblastine (concentrations 10(-6) and 10(-4) M), there was a continuous accumulation of mitoses after vinblastine was removed. At 10(-6) M, a small proportion of anaphase figures was observed in reversed cell populations but at 10(-4) M, there was no anaphase. The results suggest that tubulin was precipitated or destroyed even in cells in interphase. The G2 and S cells were able to complete their cell cycle but were unable to divide because of the lack of microtubules. When recovery was prolonged to 24, 48 and 72 hr, the cell populations exhibited higher percentages of polyploid and aneuploid elements than the control cell populations. There appeared to be a nonrandom increase of the small metacentric than the larger chromosomes. This test system, after protocol improvements, should be useful to assay mitotic poisons that are water soluble or soluble in DMSO.
Authors:
T C Hsu; L R Shirley; H Takanari
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Anticancer research     Volume:  3     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    1983 May-Jun
Date Detail:
Created Date:  1983-08-26     Completed Date:  1983-08-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  155-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cricetinae
Cricetulus
Demecolcine / pharmacology*
Diploidy
Mitosis / drug effects*
Vinblastine / pharmacology*
Chemical
Reg. No./Substance:
477-30-5/Demecolcine; 865-21-4/Vinblastine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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