| Cytogenetic analysis of reconstituted one-cell mouse embryos derived from nuclear transfer of fetal male germ cells. | |
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MedLine Citation:
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PMID: 1432959 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Micromanipulation techniques were used to produce reconstituted one-cell mouse embryos after the fusion of fetal male germ cells 15.5 day post coitum with enucleated secondary oocytes. At this stage of development, male fetal germ cells are arrested at G1 of mitotic interphase. Two distinct populations of germ cells, differing in size and ploidy, were isolated from the genital ridge of a mid-term fetus. Oocytes that had received male germ cells from the population of smaller (mononuclear) germ cells developed as diploid one-cell reconstituted embryos. When the same procedures were used to produce reconstituted one-cell embryos using male fetal germ cells from a population of larger (multinucleate) cells, they exhibited ploidy of either 4x, 6x or 8x at metaphase of the first cell division. Although most reconstituted embryos (90 and 96%) developed to the two-cell stage, the proportion of embryos receiving small germ cells developed to blastocysts was much higher (62%) than that receiving large germ cells (4%). These studies indicate that not all fetal germ cells are diploid before the onset of meiosis and have identified procedures to produce reconstituted embryos from fetal germ cells that do not carry genome or chromosome anomalies. |
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Authors:
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Y Tsunoda; Y Kato; G T O'Neill |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of reproduction and fertility Volume: 96 ISSN: 0022-4251 ISO Abbreviation: J. Reprod. Fertil. Publication Date: 1992 Sep |
Date Detail:
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Created Date: 1992-12-18 Completed Date: 1992-12-18 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376367 Medline TA: J Reprod Fertil Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 275-81 Citation Subset: IM |
Affiliation:
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Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Size / physiology Chromosomes / physiology* Cleavage Stage, Ovum / physiology Embryo, Mammalian / physiology* Embryonic and Fetal Development / physiology Female Karyotyping Male Mice Mice, Inbred Strains Nuclear Transfer Techniques Ploidies Spermatozoa / cytology, physiology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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