| Cytogenetic analysis of embryos generated from in vitro matured mouse oocytes reveals an increase in micronuclei due to chromosome fragmentation. | |
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MedLine Citation:
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PMID: 11958507 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: (i) To determine the prevalence of micronuclei in the cytoplasm of embryos generated from in vitro matured oocytes. (ii) Assess whether micronuclei presence are the result of chromosome fragmentation or the loss of whole chromosomes. METHODS: In vitro fertilization was performed on mature oocytes generated from superovulated mice (control) and in vitro matured mouse oocytes. Fertilized oocytes were cultured to the two-cell stage and fixed to slides. Micronuclei assessment was performed after staining with Giemsa. Centromere assessment was made using immunofluorescent staining (CREST) of the centromeric kinetochores. RESULTS: Micronuclei were observed in 2% (4/197) of control two-cell embryos and 36.2% (46/127) of two-cell embryos generated from in vitro matured oocytes (P < 0.02). Centromeres were not detected in micronuclei from either group. CONCLUSIONS: A significant increase in micronuclei was observed in embryos generated from in vitro matured oocytes. The lack of accompanyingcentromeres would suggest the micronuclei are the result of chromosome fragmentation. |
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Authors:
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S M Junk; A R Murch; A Dharmarajan; J L Yovich |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of assisted reproduction and genetics Volume: 19 ISSN: 1058-0468 ISO Abbreviation: J. Assist. Reprod. Genet. Publication Date: 2002 Feb |
Date Detail:
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Created Date: 2002-04-17 Completed Date: 2002-10-11 Revised Date: 2013-02-19 |
Medline Journal Info:
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Nlm Unique ID: 9206495 Medline TA: J Assist Reprod Genet Country: United States |
Other Details:
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Languages: eng Pagination: 67-71 Citation Subset: IM |
Affiliation:
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PIVET Medical Centre, Leederville, Western Australia, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Centromere / genetics Chromosome Breakage* Cytogenetic Analysis Embryo, Mammalian / physiology* Female Fertilization in Vitro* Male Mice Mice, Inbred Strains Micronuclei, Chromosome-Defective* Oocytes / physiology* |
| Comments/Corrections | |
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