Document Detail

Cytogenetic alterations in buccal mucosa cells of chewers of areca nut and tobacco.
MedLine Citation:
PMID:  20863483     Owner:  NLM     Status:  In-Process    
OBJECTIVE: the rationale of the study was to evaluate the cytological alterations especially micronucleus (MN) and other nuclear anomalies in buccal mucosa cells of chewers to understand the genotoxic and clastogenic potential of chewing mixture (containing areca nut and tobacco as main ingredients).
METHODS: the buccal cytome assay involves the examination of epithelial smear to determine micronucleated cell and other nuclear anomalies after the Feulgen plus light green staining. The assay was applied to exfoliated buccal mucosa cells of 262 subjects [non-chewers - 161 and chewers - 101 (includes 20 subjects with OSMF)] and 1000 cells per individual were examined microscopically. Nuclear anomalies were compared among chewers, non-chewers and OSMF subjects and correlated with consumption of quids per day and duration of chewing in years.
RESULTS: MN cells were found significantly (p<0.0001) higher among chewers and OSMF subjects as compared to non-chewers. Further analysis indicated that MN was significantly higher in OSMF subjects with respect to even chewers. Nuclear buds were significantly higher (p<0.0001) in OSMF subjects as compared to chewers as well as non-chewers. Nuclear anomalies viz. binucleated, karyorrhexis and karyolysis were also considerably higher in OSMF subjects as compared to non-chewers.
CONCLUSION: the MN and other nuclear anomalies reflected genetic damage and cytotoxicity, associated with tobacco and areca nut consumption. Further, these data reveal a risk for development of OSMF among chewers of mixture containing areca nut and/or tobacco, as all the OSMF subjects were chewers.
M S Joshi; Y Verma; A K Gautam; G Parmar; B C Lakkad; Sunil Kumar
Related Documents :
21116413 - Marine benthic cyanobacteria contain apoptosis-inducing activity synergizing with dauno...
16915513 - Histone h3 variants in male gametic cells of lily and h3 methylation in mature pollen.
17549403 - Insulin-like growth factor-i has different effects on myogenin induction and cell cycle...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-22
Journal Detail:
Title:  Archives of oral biology     Volume:  56     ISSN:  1879-1506     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  63-7     Citation Subset:  D; IM    
Copyright Information:
2010 Elsevier Ltd. All rights reserved.
Division of Reproductive and Cytotoxicology, National Institute of Occupational Health (ICMR), Ahmedabad 380 016, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Presence of Helicobacter pylori in supragingival dental plaque of individuals with periodontal disea...
Next Document:  Glycan structures and antiviral effect of the structural subunit RvH2 of Rapana hemocyanin.