Document Detail

Cytogenetic abnormalities in exfoliated oral mucosal cells and their association with oral cancer.
MedLine Citation:
PMID:  22611754     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To assess cytogenetic abnormalities through quantification of micronuclei, broken eggs, and karyorrhexis, in cells from normal oral mucosa of individuals exposed to carcinogens (alcohol and tobacco) and adjacent to leukoplakias and squamous cell carcinomas.
STUDY DESIGN: The sample was composed of 40 subjects aged > 30 years, divided into four groups: control, alcohol/tobacco, leukoplakia, and squamous cell carcinoma. For control and alcohol/tobacco groups, cells were collected from lower lip, tongue border, and floor of the mouth. For leukoplakia and squamous cell carcinoma groups, mucosa contralateral and adjacent to the lesions were analyzed. Cytologic smears were stained with the Feulgen reaction. A blind observer analyzed 1,000 cells per slide to quantify micronuclei, broken eggs, and karyorrhexis.
RESULTS: The leukoplakia group showed an increased number of micronuclei compared to controls (p = 0.0016) and the alcohol/tobacco group (p = 0.0048) and also increased broken eggs compared to the alcohol/tobacco group (p = 0.0172). Similarly, the carcinoma group presented more micronuclei compared to controls (p = 0.0462) and more broken eggs compared to the alcohol/tobacco group (p = 0.0104).
CONCLUSION: The assessment of cytogenetic abnormalities micronuclei and broken eggs may be useful for monitoring individuals exposed to risk factors for developing oral cancer.
Ana Carolina Amorim Pellicioli; Fernanda Visioli; Luciana Adolfo Ferreira; Chris Krebs Danilevicz; Vinicius Coelho Carrard; Pantelis Varvaki Rados
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology     Volume:  33     ISSN:  0884-6812     ISO Abbreviation:  Anal. Quant. Cytol. Histol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2012-05-22     Completed Date:  2012-06-12     Revised Date:  2014-11-05    
Medline Journal Info:
Nlm Unique ID:  8506819     Medline TA:  Anal Quant Cytol Histol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  271-6     Citation Subset:  IM    
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MeSH Terms
Alcohol Drinking / genetics,  pathology
Carcinoma, Squamous Cell / genetics*,  pathology
Cell Nucleus / pathology
Chromosome Aberrations*
Leukoplakia, Oral / genetics*,  pathology
Micronuclei, Chromosome-Defective
Micronucleus Tests
Mouth Mucosa / pathology,  physiology*
Mouth Neoplasms / genetics*,  pathology
Smoking / genetics,  pathology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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