Document Detail


Cytochrome P450 expression and catalytic activity in coronary arteries and liver of cattle.
MedLine Citation:
PMID:  15716024     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There is increasing evidence that cytochrome P450 (CYP) enzymes are involved not only in the metabolism of xenobiotics, but also in vascular homeostasis. Among the CYP-derived vasoactive agents, special importance is assigned to endogenous products from arachidonic acid (AA). Specifically, the vasodilator epoxyeicosatrienoic acids (EETs), being linked to the CYP 2B, 2C, and 2J subfamilies, and the vasoconstrictor 20-hydroxyeicosatetraenoic acid (20-HETE) connected instead to the CYP 4A subfamily and, to a lesser degree, to isoforms of the CYP 1A and 2E subfamilies. Here, we have examined the occurrence of functional CYP isoforms in the coronary arteries of cattle by RT-PCR with sequence verification, Western immunoblotting, and analysis of distinct catalytic activities with fluorescent substrate probes. Liver tissue was examined comparatively. Coronary tissue expressed mRNA transcripts and immunoreactive proteins belonging to the CYP 1A, 2C, 2E, and 2J subfamilies. Appropriate catalytic activity was ascertained with all these CYP species except 2J. A broader spectrum of CYP enzymes (CYP 1A, 2B, 2C, 2D, 2E, 2J, 3A, 4A subfamilies) was found in liver tissue with catalytic activities exceeding many fold those of coronary tissue. We conclude that bovine coronary arteries are endowed with a full-fledged CYP system with potential for AA-linked vasoregulation through dilator rather than constrictor agents. The same tissue and, to a much larger degree, liver tissue possess the capability of metabolizing xenobiotics via the CYP pathway.
Authors:
Emanuela Grasso; Vincenzo Longo; Flavio Coceani; Pier Giovanni Gervasi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-12-31
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1722     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-04-12     Completed Date:  2005-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  116-23     Citation Subset:  IM    
Affiliation:
Scuola Superiore S. Anna, Italy.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cattle
Coronary Vessels / enzymology*
Cytochrome P-450 Enzyme System / genetics*,  metabolism
DNA Primers
Isoenzymes / genetics,  metabolism
Liver / enzymology*
Microsomes, Liver / enzymology
Molecular Sequence Data
Polymerase Chain Reaction
Sequence Alignment
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Sheep
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Isoenzymes; 9035-51-2/Cytochrome P-450 Enzyme System

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