Document Detail


Cytochrome P450 family member CYP704B2 catalyzes the {omega}-hydroxylation of fatty acids and is required for anther cutin biosynthesis and pollen exine formation in rice.
MedLine Citation:
PMID:  20086189     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The anther cuticle and microspore exine act as protective barriers for the male gametophyte and pollen grain, but relatively little is known about the mechanisms underlying the biosynthesis of the monomers of which they are composed. We report here the isolation and characterization of a rice (Oryza sativa) male sterile mutant, cyp704B2, which exhibits a swollen sporophytic tapetal layer, aborted pollen grains without detectable exine, and undeveloped anther cuticle. In addition, chemical composition analysis indicated that cutin monomers were hardly detectable in the cyp704B2 anthers. These defects are caused by a mutation in a cytochrome P450 family gene, CYP704B2. The CYP704B2 transcript is specifically detected in the tapetum and the microspore from stage 8 of anther development to stage 10. Heterologous expression of CYP704B2 in yeast demonstrated that CYP704B2 catalyzes the production of omega -hydroxylated fatty acids with 16 and 18 carbon chains. Our results provide insights into the biosynthesis of the two biopolymers sporopollenin and cutin. Specifically, our study indicates that the omega -hydroxylation pathway of fatty acids relying on this ancient CYP704B family, conserved from moss to angiosperms, is essential for the formation of both cuticle and exine during plant male reproductive and spore development.
Authors:
Hui Li; Franck Pinot; Vincent Sauveplane; Danièle Werck-Reichhart; Patrik Diehl; Lukas Schreiber; Rochus Franke; Ping Zhang; Liang Chen; Yawei Gao; Wanqi Liang; Dabing Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-19
Journal Detail:
Title:  The Plant cell     Volume:  22     ISSN:  1532-298X     ISO Abbreviation:  Plant Cell     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-03-04     Completed Date:  2010-04-15     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  173-90     Citation Subset:  IM    
Affiliation:
School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Biopolymers / biosynthesis
Carotenoids / biosynthesis
Cloning, Molecular
Cytochrome P-450 Enzyme System / genetics,  metabolism*
Fatty Acids / metabolism*
Gene Expression Regulation, Plant
Genetic Complementation Test
Hydroxylation
Membrane Lipids / biosynthesis*
Molecular Sequence Data
Mutation
Oryza sativa / enzymology*,  genetics,  growth & development
Phylogeny
Plant Proteins / genetics,  metabolism*
Pollen / growth & development*
RNA, Plant / genetics
Recombinant Proteins / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Biopolymers; 0/Fatty Acids; 0/Membrane Lipids; 0/Plant Proteins; 0/RNA, Plant; 0/Recombinant Proteins; 12712-72-0/sporopollenin; 36-88-4/Carotenoids; 54990-88-4/cutin; 9035-51-2/Cytochrome P-450 Enzyme System
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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