| Cytochrome P-450 localization in normal human adult and foetal liver by immunocytochemistry using a monoclonal antibody against human cytochrome P-450. | |
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MedLine Citation:
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PMID: 3440754 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immunocytochemical studies with a monoclonal antibody (MAb-HL3), which recognises a major isozyme of human hepatic cytochrome P-450, have demonstrated this cytochrome in both cryostat and formalin-fixed paraffin-embedded sections of normal human adult liver. Prior trypsin digestion of the formalin-fixed sections prevented staining. There was a zonal distribution of immunoreactive cytochrome P-450, with localization predominantly in the hepatocytes of zone 3 of the hepatic acinus (the centrilobular region). Cytochrome P-450 was also demonstrated in foetal liver, but all foetal hepatocytes contained immunoreactive cytochrome P-450 and there was no zonal distribution of the protein. The biliary epithelium of adult liver contained a small amount of immunoreactive cytochrome P-450 whereas there was no immunoreactivity in the epithelium of foetal bile ducts. |
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Authors:
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G I Murray; T S Barnes; H F Sewell; S W Ewen; W T Melvin; P M Shaw; J Fowler; M D Burke |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Histochemical journal Volume: 19 ISSN: 0018-2214 ISO Abbreviation: Histochem. J. Publication Date: 1987 Oct-Nov |
Date Detail:
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Created Date: 1988-04-18 Completed Date: 1988-04-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0163161 Medline TA: Histochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 537-45 Citation Subset: IM |
Affiliation:
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Department of Pathology, University of Aberdeen, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antibodies, Monoclonal / diagnostic use* Cytochrome P-450 Enzyme System / analysis*, immunology Fetus / enzymology* Humans Immunohistochemistry Liver / enzymology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 9035-51-2/Cytochrome P-450 Enzyme System |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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