Document Detail


Cytochemistry and ultrastructure of canine myocardium undergoing global ischemia and reperfusion injury.
MedLine Citation:
PMID:  10063788     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to highlight severity of myocardial injury during the course of global ischemia and reperfusion, cytochemistry of glycogen and succinate dehydrogenase (SDH) as well as hematoxylin and eosin staining (H & E) and electron microscopy were observed in canine myocardium. Seven mongrel dogs were selected for reperfusion injury after global ischemia in this study. Myocardial biopsies were taken from the anterior wall of the left ventricle (a) after cardiopulmonary bypass (the first biopsy); (b) at the end of the aortic crossclamp (the second biopsy); and (c) 30 minutes after crossclamp removal (the third biopsy). All biopsies were cytochemically assessed, and the latter two, for electron microscopic studies. The averages of myocardial necrotic rate and surface to volume ratio of myocardial mitochondria were calculated under electron microscopy and in electron microscopic slices, respectively. Myofibrillae were of normal morphology in the first biopsy; in wave-shape and partly vacuolated, with large and deformed nuclei in the second one; and in wave-shape and severely vacuolated in the third one, in H & E. Glycogen granules were variously stained in moderate, weak and intensive positive reactions in the three biopsies respectively in glycogen staining. SDH was stained in intensive, weak, and moderate positive reactions in three, respectively. By electron microscopy, Z bands twisted severely, and local dissolution of cristae and matrix occurred in a minority of the mitochondria in the second biopsy; and majority of the Z bands in necrotic region had disappeared, the myofibrillae were obscure and patchily dissolved. Clustered and deformed mitochondria could be found in the third biopsy. Significant difference could be noted between the averages of the second and third biopsies (14.88 +/- 3.09% vs. 60.25 +/- 8.55%, p < 0.001). The surface to volume ratio of the ischemic mitochondria was much bigger than that of the reperfused (3.95 +/- 1.09 micron-1 vs. 2.77 +/- 0.93 micron-1, p = 0.041). Myocardial injury was more severe in reperfusion than in ischemia myocardium. There were correlations between histobiochemical and ultrastructural alterations in damaged canine myocardium.
Authors:
S M Yuan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Kaohsiung journal of medical sciences     Volume:  15     ISSN:  1607-551X     ISO Abbreviation:  Kaohsiung J. Med. Sci.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-03-16     Completed Date:  1999-03-16     Revised Date:  2006-08-28    
Medline Journal Info:
Nlm Unique ID:  100960562     Medline TA:  Kaohsiung J Med Sci     Country:  TAIWAN    
Other Details:
Languages:  eng     Pagination:  1-7     Citation Subset:  IM    
Affiliation:
Department of Cardiothoracic Surgery, 254 Hospital of Tianjin, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Dogs
Glycogen / analysis*
Microscopy, Electron
Mitochondria, Heart / ultrastructure
Myocardial Ischemia / metabolism*,  pathology
Myocardial Reperfusion Injury / metabolism*,  pathology
Myocardium / ultrastructure*
Succinate Dehydrogenase / metabolism*
Chemical
Reg. No./Substance:
9005-79-2/Glycogen; EC 1.3.99.1/Succinate Dehydrogenase

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