Document Detail


Cytochemical demonstration of mercury deposits in trout liver and kidney following methyl mercury intoxication: differentiation of two mercury pools by selenium.
MedLine Citation:
PMID:  3691368     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The amount and the ultrastructural distribution of mercury was studied in seven different organs of rainbow trout (Salmo gairdneri) fingerlings following exposure to methyl mercury (MeHg)-contaminated fodder for periods of 2 and 7 weeks. The amounts of mercury retained by the whole fish and the selected organs were determined by measuring the uptake of 203Hg-labeled MeHg. Spleen, liver, and kidney had the highest concentrations after both experimental periods, while the largest relative increases were found in brain, muscle, and kidney. The subcellular distribution of mercury accumulations was demonstrated cytochemically in liver and kidney using the silver enhancement method by which accumulations of mercury-sulfides and/or mercury-selenides are made visible for light and electron microscopy. When sections prepared from the liver and kidney from fish, injected with selenium 2 hr prior to being killed, were compared with those of fish not treated with selenium, two distinct pools of mercury could be demonstrated, the HgS pool, and the HgSe pool. The HgS pool, supposed to represent inorganic mercury, was found exclusively within lysosomes. The increase of this pool from 2 to 7 weeks was most pronounced in the kidney. The HgSe pool, supposed to represent methyl mercury, was shown by the presence of silver deposits at new locations as well as by an increase in the amount of deposits within lysosomes. The new locations included (1) secretory-like vesicles and the bile canaliculi of the liver, suggesting a biliary excretion of this mercury pool; (2) microvilli and endosomes of kidney tubular cells, suggesting a glomerular filtration and subsequent reabsorption; and (3) mitochondria of proximal tubule cells.
Authors:
E Baatrup; G Danscher
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Ecotoxicology and environmental safety     Volume:  14     ISSN:  0147-6513     ISO Abbreviation:  Ecotoxicol. Environ. Saf.     Publication Date:  1987 Oct 
Date Detail:
Created Date:  1988-01-26     Completed Date:  1988-01-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7805381     Medline TA:  Ecotoxicol Environ Saf     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  129-41     Citation Subset:  IM    
Affiliation:
Institute of Zoology and Zoophysiology, University of Aarhus, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Animals
Histocytochemistry
Kidney / metabolism*,  ultrastructure
Liver / metabolism*
Mercury / metabolism*
Mercury Radioisotopes / diagnostic use
Methylmercury Compounds / metabolism*,  poisoning
Microscopy, Electron
Salmonidae / metabolism*
Selenium / diagnostic use*
Silver
Trout / metabolism*
Chemical
Reg. No./Substance:
0/Mercury Radioisotopes; 0/Methylmercury Compounds; 7439-97-6/Mercury; 7440-22-4/Silver; 7782-49-2/Selenium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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