Document Detail

Cytochemical demonstration of the glucocorticoid receptor in skeletal cells of the rat.
MedLine Citation:
PMID:  10098598     Owner:  NLM     Status:  MEDLINE    
The in vivo localization of the glucocorticoid receptor (GR) was studied in cartilage and bone cells of femurs of young adult rats. Deparaffinized sections were treated with a polyclonal antibody raised against the amino-terminus of human GR; the immunoreaction was detected with the streptavidin-biotin amplification method. Histomorphometric, computer-assisted analysis of GR-positive cells was performed by counting the percentage of GR-immunostained cells in the proliferative and maturative/hypertrophic zone of the epiphyseal growth plate cartilage, and of the percentage of positive osteoblasts (OBs), osteoclasts (OCLS) and osteocytes (OCs) in the metaphyseal secondary ossification zone. Numbers of OBs and OCLs per mm of metaphyseal endosteal perimeter, and numbers of OCs per mm2 of trabecular area were also counted. Immunopositive cells were found both in cartilage and bone, with variable degree of nuclear and/or cytoplasmic immunostaining; immunonegative cells were present among the positive ones. Histomorphometry showed that about 54% of chondrocytes in the proliferative zone, and 55% of chondrocytes in the maturative/hypertrophic zone of the growth plate were labeled; in metaphyseal bone, 68% of OBs, 65% of OCs, and 98% of OCLs were GR-positive. The density of positive cells was 12.06 OBs/mm, 3.32 OCLs/mm, and 520.40 OCs/mm2. These results, for the first time obtained in vivo, show that GR is present in cartilage and bone cells, and that the degree of GR-immunostaining is variable in the same type of cell. This may be dependent on the cell cycle and stage of differentiation, and may reflect a variable cellular sensitivity to the stimulation of the glucocorticoid hormone.
G Silvestrini; P Mocetti; P Ballanti; R Di Grezia; E Bonucci
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrine research     Volume:  25     ISSN:  0743-5800     ISO Abbreviation:  Endocr. Res.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-05-27     Completed Date:  1999-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8408548     Medline TA:  Endocr Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  117-28     Citation Subset:  IM    
Department of Experimental Medicine and Pathology, University La Sapienza, Rome, Italy.
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MeSH Terms
Cell Count
Femur / cytology,  metabolism*
Growth Plate / cytology,  metabolism
Osteogenesis / physiology
Rats, Sprague-Dawley
Receptors, Glucocorticoid / metabolism*
Reg. No./Substance:
0/Receptors, Glucocorticoid

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