Document Detail


Cytoarchitecture and ultrastructure of neural stem cell niches and neurogenic complexes maintaining adult neurogenesis in the olfactory midbrain of spiny lobsters, Panulirus argus.
MedLine Citation:
PMID:  21523781     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
New interneurons are continuously generated in small proliferation zones within neuronal somata clusters in the olfactory deutocerebrum of adult decapod crustaceans. Each proliferation zone is connected to a clump of cells containing one neural stem cell (i.e., adult neuroblast), thus forming a "neurogenic complex." Here we provide a detailed analysis of the cytoarchitecture of neurogenic complexes in adult spiny lobsters, Panulirus argus, based on transmission electron microscopy and labeling with cell-type-selective markers. The clump of cells is composed of unique bipolar clump-forming cells that collectively completely envelop the adult neuroblast and are themselves ensheathed by a layer of processes of multipolar cell body glia. An arteriole is attached to the clump of cells, but dye perfusion experiments show that hemolymph has no access to the interior of the clump of cells. Thus, the clump of cells fulfills morphological criteria of a protective stem cell niche, with clump-forming cells constituting the adult neuroblast's microenvironment together with the cell body glia processes separating it from other tissue components. Bromodeoxyuridine pulse-chase experiments with short survival times suggest that adult neuroblasts are not quiescent but rather cycle actively during daytime. We propose a cell lineage model in which an asymmetrically dividing adult neuroblast repopulates the pool of neuronal progenitor cells in the associated proliferation zone. In conclusion, as in mammalian brains, adult neurogenesis in crustacean brains is fueled by neural stem cells that are maintained by stem cell niches that preserve elements of the embryonic microenvironment and contain glial and vascular elements.
Authors:
Manfred Schmidt; Charles D Derby
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  519     ISSN:  1096-9861     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-06-28     Completed Date:  2011-10-11     Revised Date:  2014-01-10    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2283-319     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / metabolism
Cell Proliferation
Female
Male
Mesencephalon / cytology
Neural Stem Cells / physiology,  ultrastructure*
Neurogenesis*
Neuroglia / ultrastructure
Olfactory Pathways / anatomy & histology*,  physiology
Palinuridae / anatomy & histology*,  physiology
Stem Cell Niche*
Grant Support
ID/Acronym/Agency:
DC00312/DC/NIDCD NIH HHS; R01 DC000312/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers
Comments/Corrections

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