Document Detail

Cytidine triphosphate: phosphatidic acid cytidyltransferase in Escherichia coli.
MedLine Citation:
PMID:  4879388     Owner:  NLM     Status:  MEDLINE    
An enzyme has been found in particulate fractions of Escherichia coli that catalyzes the incorporation of cytidine triphosphate (CTP) into lipid in the presence of exogenous phosphatidic acid and Mg(++). The product has been identified enzymatically and by chromatography as cytidine diphosphate diglyceride. The reaction is optimal at a pH of 6.5 and Mg(++) concentration of 5-10 mm. The apparent K(m) for CTP is 7 x 10(-4)M and for phosphatidic acid, 2 x 10(-3)M. The reaction rate falls off rapidly with time and ceases entirely after 1 hr as the result of inactivation of the system by Mg(++).
J R Carter
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  9     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1968 Nov 
Date Detail:
Created Date:  1968-12-09     Completed Date:  1968-12-09     Revised Date:  2009-10-27    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  748-54     Citation Subset:  IM    
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MeSH Terms
Cytosine Nucleotides / metabolism
Escherichia coli / enzymology*
Glycerides / biosynthesis
Hydrogen-Ion Concentration
Lipids / biosynthesis
Nucleotidyltransferases / metabolism*
Reg. No./Substance:
0/Cytosine Nucleotides; 0/Glycerides; 0/Lipids; 0/Phospholipids; 10028-17-8/Tritium; 7439-95-4/Magnesium; EC 2.7.7.-/Nucleotidyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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