Document Detail


Cytidine triphosphate: phosphatidic acid cytidyltransferase in Escherichia coli.
MedLine Citation:
PMID:  4879388     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An enzyme has been found in particulate fractions of Escherichia coli that catalyzes the incorporation of cytidine triphosphate (CTP) into lipid in the presence of exogenous phosphatidic acid and Mg(++). The product has been identified enzymatically and by chromatography as cytidine diphosphate diglyceride. The reaction is optimal at a pH of 6.5 and Mg(++) concentration of 5-10 mm. The apparent K(m) for CTP is 7 x 10(-4)M and for phosphatidic acid, 2 x 10(-3)M. The reaction rate falls off rapidly with time and ceases entirely after 1 hr as the result of inactivation of the system by Mg(++).
Authors:
J R Carter
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  9     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1968 Nov 
Date Detail:
Created Date:  1968-12-09     Completed Date:  1968-12-09     Revised Date:  2009-10-27    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  748-54     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cytosine Nucleotides / metabolism
Escherichia coli / enzymology*
Glycerides / biosynthesis
Hydrogen-Ion Concentration
Kinetics
Lipids / biosynthesis
Magnesium
Nucleotidyltransferases / metabolism*
Phospholipids
Tritium
Vibration
Chemical
Reg. No./Substance:
0/Cytosine Nucleotides; 0/Glycerides; 0/Lipids; 0/Phospholipids; 10028-17-8/Tritium; 7439-95-4/Magnesium; EC 2.7.7.-/Nucleotidyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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