Document Detail


Cystoisospora belli: in vitro multiplication in mammalian cells.
MedLine Citation:
PMID:  16674944     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intracellular development of Cystoisospora belli was demonstrated in 4 different mammalian cell lines. Human ileocecal adenocarcinoma (HCT-8), epithelial carcinoma of lung (A549), Madin-Darby bovine kidney (MDBK), and African green monkey kidney (VERO) were exposed in vitro to C. belli sporozoites, which had been isolated from the feces of HIV-AIDS patients. Parasites invaded all the cellular types between 4 and 12h after exposure and multiplication was demonstrated after 24 h. Grater number of merozoites formed in VERO cells, followed by HCT-8. In the MDBK and HCT-8 cells, the parasitophorous vacuole was less evident and immobile merozoites were observed in the cytoplasm. In VERO cells, one or several parasitophorous vacuoles contained up to 16 mobile sporozoites. No oocysts were found in any of the cell types used. VERO cells may be suitable for studies of the interaction between parasite and host cells.
Authors:
M B Oliveira-Silva; E Lages-Silva; D V Resende; A Prata; L E Ramirez; J K Frenkel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-03
Journal Detail:
Title:  Experimental parasitology     Volume:  114     ISSN:  0014-4894     ISO Abbreviation:  Exp. Parasitol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-18     Completed Date:  2006-11-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370713     Medline TA:  Exp Parasitol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  189-92     Citation Subset:  IM    
Affiliation:
Departamento Ciências Biológicas-Disciplina de Parasitologia, Universidade Federal do Triângulo Mineiro, Rua Frei Paulino, 30, 38025-180 Uberaba, MG, Brazil. mbosilva@yahoo.com.br <mbosilva@yahoo.com.br>
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MeSH Terms
Descriptor/Qualifier:
AIDS-Related Opportunistic Infections / complications,  parasitology*
Animals
Cattle
Cell Line
Cell Line, Tumor
Cercopithecus aethiops
Coccidiosis / complications,  parasitology*
Feces / parasitology
Humans
Sarcocystidae / growth & development*
Vero Cells

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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