Document Detail


Cystine requirement of continuous human lymphoid cell lines of normal and leukemic origin.
MedLine Citation:
PMID:  903356     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A number of continuous, human lymphoid cell lines from healthy and leukemic donors have been studied. Those from healthy individuals were found to be cystine prototrophs (cys+), while those from leukemic donors were observed to be cystine auxotrophs (cys-). The former can grow in either cystine or cystathionine-containing media. The latter grow only in cystine-containing medium. The former contain readily measurable levels of cystathionase, the last enzyme in the mammalian cysteine biosynthetic pathway, while the latter contain much less of this activity. The apparent molecular weight, overall charge, pH versus activity, Km, and [pyridoxal phosphate] versus activity properties of the enzyme from both cell types were indistinguishable. No soluble cystathionase inhibitor could be detected in cys- cell lines. Thus, it is reasonable to hypothesize that those leukemic cell lines studied synthesize less active cystathionase protein than their cys+ counterparts and that this difference in enzyme production underlies the differential growth properties in cystathionine-containing medium.
Authors:
J K Iglehart; R M York; A P Modest; H Lazarus; D M Livingston
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  252     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1977 Oct 
Date Detail:
Created Date:  1977-11-25     Completed Date:  1977-11-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7184-91     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Culture Media
Cystathionine gamma-Lyase / metabolism
Cystine / metabolism*
Enzyme Inhibitors / isolation & purification
Humans
Isoelectric Focusing
Kinetics
Leukemia / metabolism*
Lymphocytes / metabolism*
Mice
Molecular Weight
Rats
Chemical
Reg. No./Substance:
0/Culture Media; 0/Enzyme Inhibitors; 56-89-3/Cystine; EC 4.4.1.1/Cystathionine gamma-Lyase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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