Document Detail

Cystine and lysine reabsorption in the isolated perfused rat kidney.
MedLine Citation:
PMID:  2566279     Owner:  NLM     Status:  MEDLINE    
Renal tubular reabsorption of cystine and lysine were studied in the isolated perfused rat kidney to bridge the gap between in vivo clearance studies, and in vitro transport studies of tubule fragments, cells, and brush-border membranes. Lysine was reabsorped by a saturable transport system shared by the dibasics. Cystine was also reabsorbed by a saturable transport system, which was shared in part by the dibasics (maximum inhibition 30%). The lysine threshold (Fmin) was 0.9 mumol.min-1.g-1, with a tubular maximum (TM) of 2.4 mumol.min-1.g-1. The cystine Fmin was 0.06 mumol.min-1.g-1; the TM could not be estimated because it was above the limit of cystine solubility. There was no evidence of cystine "secretion." The gamma-glutamyltransferase inhibitor, AT-125, decreased cystine excretion, but only in the presence of glutathione, glycine, glutamate, and the diabasic amino acids. This suggests that cystine from glutathione degradation at the brush border may contribute to urinary cystine (an explanation of the phenomenon of cystine secretion), but only under certain conditions.
K A Roby; S Segal
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The American journal of physiology     Volume:  256     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-06-16     Completed Date:  1989-06-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  F901-8     Citation Subset:  IM    
Division of Biochemical Development and Molecular Diseases, Children's Hospital of Philadelphia, Pennsylvania.
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MeSH Terms
Amino Acids / pharmacology
Cystine / pharmacokinetics*
Glutathione / pharmacology
Isoxazoles / pharmacology
Kidney / metabolism*
Lysine / pharmacokinetics*
Rats, Inbred Strains
gamma-Glutamyltransferase / antagonists & inhibitors
Reg. No./Substance:
0/Amino Acids; 0/Isoxazoles; 52583-41-2/acivicin; 56-87-1/Lysine; 56-89-3/Cystine; 70-18-8/Glutathione; EC

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