Document Detail


Cystine and dibasic amino acid uptake by opossum kidney cells.
MedLine Citation:
PMID:  2358474     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The characteristics of the uptake of L-cystine by the continuous opossum kidney cell line, OK, were examined. Uptake of cystine is rapid and, in contrast to other continuous cultured cell lines, these cells retain the cystine/dibasic amino acid transport system which is found in vivo and in freshly isolated kidney tissue. Confluent monolayers of cells also fail to show the presence of the cystine/glutamate transport system present in LLC-PK1 cells, fibroblasts, and cultured hepatocytes. Uptake of cystine occurs via a high-affinity saturable process which is independent of medium sodium concentration. The predominant site of cystine transport is across the apical cell membrane. The intracellular concentration of GSH far exceeds that of cystine with a ratio greater than 100:1 for GSH:cysteine. Incubation of cells for 5 minutes with a physiological level of labelled cystine resulted in the labelling of 66% and 5% of the total intracellular cysteine and glutathione, respectively. The ability of these cells to reflect the shared cystine/dibasic amino acid transport system makes them a suitable model for investigation of the cystine carrier which is altered in human cystinuria.
Authors:
B States; S Segal
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  143     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-07-27     Completed Date:  1990-07-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  555-62     Citation Subset:  IM    
Affiliation:
Division of Biochemical Development and Molecular Diseases, Children's Hospital of Philadelphia, Pennsylvania.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism,  pharmacokinetics*
Animals
Cell Line
Cells, Cultured
Cystine / metabolism,  pharmacokinetics*
Dose-Response Relationship, Drug
Kidney / cytology*,  metabolism
Opossums / metabolism*
Sodium / pharmacology
Sulfur Radioisotopes / diagnostic use
Grant Support
ID/Acronym/Agency:
DK40555/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Sulfur Radioisotopes; 56-89-3/Cystine; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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