Document Detail

Cysteine string protein beta is prominently associated with nerve terminals and secretory organelles in mouse brain.
MedLine Citation:
PMID:  20338151     Owner:  NLM     Status:  MEDLINE    
Cysteine string proteins (CSPs) are associated with regulated secretory organelles in organisms ranging from fruit flies to man. Mammals have three csp genes (alpha, beta and gamma), and previous work indicated that expression of the csp-beta and -gamma genes was restricted to the testes. For the current investigation, antibodies specific for CSP-beta were developed. Unexpectedly, immunoblot analysis indicated that CSP-beta was prominently expressed throughout mouse brain. Upon sub-cellular fractionation, CSP-beta was enriched in synaptosomes and synaptic vesicle fractions. Interestingly, CSP-beta existed almost exclusively as part of a high mass complex both in testis and brain. This complex required aggressive denaturation to release monomeric CSP-beta. By Northern analysis CSP-beta mRNA was present at very low abundance as a approximately 1.0kb species in mouse brain. Collectively, the enrichment of CSP-beta in synaptosomes and the association of CSP-beta with synaptic vesicles suggest that CSP-beta, like CSP-alpha, may be an important component of the regulated secretory machinery in mouse brain.
Cameron B Gundersen; Sirus A Kohan; Puneet Souda; Julian P Whitelegge; Joy A Umbach
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-23
Journal Detail:
Title:  Brain research     Volume:  1332     ISSN:  1872-6240     ISO Abbreviation:  Brain Res.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-07     Completed Date:  2010-08-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1-11     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
The Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
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MeSH Terms
Amino Acid Sequence
Blotting, Northern
Brain / metabolism*
HSP40 Heat-Shock Proteins / genetics,  metabolism*
Heart Ventricles / metabolism
Membrane Proteins / genetics,  metabolism*
Mice, Inbred C57BL
Molecular Sequence Data
Neurons / metabolism*
RNA, Messenger / metabolism
Synapses / metabolism*
Synaptic Vesicles / metabolism*
Synaptosomes / metabolism*
Testis / metabolism
Reg. No./Substance:
0/HSP40 Heat-Shock Proteins; 0/Membrane Proteins; 0/RNA, Messenger; 0/cysteine string protein

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