Document Detail

Cysteine is essential for transcriptional regulation of the sulfur assimilation genes in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  10821189     Owner:  NLM     Status:  MEDLINE    
Transcription of the genes for sulfur assimilation and methionine biosynthesis in Saccharomyces cerevisiae is regulated by the size of the intracellular pool of an organic sulfur compound. The identity of this compound is not clear, but suggestions include S-adenosylmethionine (SAM) and cysteine. By studying the repression of selected sulfur assimilation (MET) genes, we found that the ability to form cysteine from homocysteine is crucial for methionine-mediated repression to take place. The transcription of MET14 and MET25 could not be repressed by methionine in strains in which either STR4 (which encodes cystathionine beta-synthase) or STR1 (cystathionine gamma-lyase) was disrupted, whereas the repression was independent of GSH1 (which encodes the enzyme responsible for the first step in glutathione biosynthesis from cysteine). In contrast, cysteine could repress the MET genes in all of these strains. Two genes that presumably encode cystathionine gamma-synthase and cystathionine beta-lyase were identified by genetic disruption (ORFs YJR130c and YGL184c), yielding yeast strains that cannot convert cysteine into homocysteine. Repression by cysteine was possible in either disruptant, suggesting a role in repression for cysteine alone. While some repression of MET genes could be accomplished by homocysteine in a strain that cannot form SAM from methionine, a low intracellular level of SAM seems to be necessary for full cysteine-mediated repression to take place.
J Hansen; P F Johannesen
Related Documents :
22123829 - Pathvar: analysis of gene and protein expression variance in cellular pathways using mi...
22084569 - The primary cilium as a biomarker in the hypoxic adaptation of bone marrow-derived mese...
22384239 - Pcagopromoter--an r package for biological and regulatory interpretation of principal c...
21934119 - Molecular characterization of 60 isolated wheat myb genes and analysis of their express...
10601209 - Prr1, a homolog of aspergillus nidulans palf, controls ph-dependent gene expression and...
10601199 - Molecular analysis of the candida albicans homolog of saccharomyces cerevisiae mnn9, re...
14707169 - Elevated rates of protein secretion, evolution, and disease among tissue-specific genes.
12925359 - Support of progress in clinical neurology by models of genetic regulation.
17031559 - Plxdc1 (tem7) is identified in a genome-wide expression screen of glioblastoma endothel...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  263     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-06-16     Completed Date:  2000-06-16     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  535-42     Citation Subset:  IM    
Carlsberg Research Laboratory, Copenhagen, Valby, Denmark.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blotting, Northern
Carbon-Oxygen Lyases / genetics
Cysteine / physiology*
Cysteine Synthase
Gene Deletion
Gene Expression Regulation, Fungal*
Models, Genetic
Multienzyme Complexes*
Open Reading Frames
S-Adenosylmethionine / genetics
Saccharomyces cerevisiae / genetics*,  physiology*
Saccharomyces cerevisiae Proteins*
Sulfur / metabolism*
Transcription, Genetic*
Reg. No./Substance:
0/Multienzyme Complexes; 0/Saccharomyces cerevisiae Proteins; 29908-03-0/S-Adenosylmethionine; 52-90-4/Cysteine; 7704-34-9/Sulfur; EC Synthase; EC protein, S cerevisiae; EC (thiol)-lyase; EC 4.2.-/Carbon-Oxygen Lyases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Functional specificity of the mitochondrial DnaJ protein, Mdj1p, in Saccharomyces cerevisiae.
Next Document:  Duplicated gene clusters suggest an interplay of glycogen and trehalose metabolism during sequential...