Document Detail


Cysteine cathepsins in human silicotic bronchoalveolar lavage fluids.
MedLine Citation:
PMID:  16303289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mature, active cysteine cathepsins (CPs) were identified in human inflammatory bronchoalveolar lavage fluid (BALF) supernatants from patients suffering from silicosis by both western blot and surface plasmon resonance analyses. BALFs are not a reservoir of activatable proforms, since no autocatalytic maturation at acidic pH occurs. Cathepsin H is the most profuse among studied CPs (median value: 36.5 nM), while cathepsins B and L are the two most abundant thiol-dependent endoproteases. The overall concentration of active cathepsins B, H, K, L, and S is approximately 10-fold lower than their concentration in BALF supernatants from patients suffering from inflammatory acute lung injuries (962+/-347 nM).The cathepsins (approximately 70 nM)/cystatin-like inhibitors (approximately 9 nM) ratio is unbalanced in favor of enzymes ( approximately 8-fold). This presence of uncontrolled CPs suggests that they may contribute, in addition to matrix metalloproteases, to the lung tissue breakdown/remodeling occurring during silicosis, although their exact contribution to interstitial inflammation remains to be evaluated.
Authors:
Céline Perdereau; Emmanuel Godat; Marie-Christine Maurel; Eric Hazouard; Elisabeth Diot; Gilles Lalmanach
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-11-07
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1762     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-06     Completed Date:  2006-04-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  351-6     Citation Subset:  IM    
Affiliation:
INSERM U618, Protéases et Vectorisation Pulmonaires, Equipe Protéases et Pathologies Pulmonaires, Université François Rabelais, Faculté de Médecine, 10 Boulevard Tonnellé, F-37032 Tours Cedex, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bronchoalveolar Lavage Fluid / chemistry*,  cytology
Cathepsins* / chemistry,  metabolism
Cysteine / metabolism*
Humans
Protein Isoforms / chemistry,  metabolism
Silicosis / metabolism*
Surface Plasmon Resonance
Chemical
Reg. No./Substance:
0/Protein Isoforms; 52-90-4/Cysteine; EC 3.4.-/Cathepsins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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