| Cysteine cathepsins are not involved in Fas/CD95 signalling in primary skin fibroblasts. | |
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MedLine Citation:
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PMID: 17950288 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The potential role of cysteine cathepsins, especially cathepsin B, in Fas/CD95-induced apoptosis was investigated using wild-type and cathepsin B-deficient primary skin fibroblasts. Apoptosis was induced with an anti-Fas/CD95 antibody in the presence of cycloheximide and no difference was observed between the two genotypes. First cells with damaged mitochondria were observed approximately 3h post apoptosis induction and their number was significantly increased after 11h. In contrast, cells with damaged lysosomes were only seen after 15h with no difference between the two genotypes. Moreover, Bid cleavage was found to be diminished in cathepsin B-deficient cells. These results suggest that cysteine cathepsins have no active role in Fas/CD95 apoptosis. |
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Authors:
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Lea Bojic; Ana Petelin; Veronika Stoka; Thomas Reinheckel; Christoph Peters; Vito Turk; Boris Turk |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-10-12 |
Journal Detail:
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Title: FEBS letters Volume: 581 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-11-05 Completed Date: 2008-01-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 5185-90 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Molecular and Structural Biology, J. Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD95 / metabolism* Apoptosis / drug effects, physiology BH3 Interacting Domain Death Agonist Protein / metabolism Cathepsin B / deficiency, genetics, metabolism* Cells, Cultured Cycloheximide / pharmacology Fibroblasts / drug effects, metabolism* Lysosomes / metabolism Mice Mice, Knockout Mitochondria / metabolism Signal Transduction Skin / cytology, drug effects, metabolism* Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD95; 0/BH3 Interacting Domain Death Agonist Protein; 0/Bid protein, mouse; 0/Fas protein, mouse; 66-81-9/Cycloheximide; EC 3.4.22.1/Cathepsin B |
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