| Cysteine cathepsins in human carious dentin. | |
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MedLine Citation:
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PMID: 21248362 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Matrix metalloproteinases (MMPs) are important in dentinal caries, and analysis of recent data demonstrates the presence of other collagen-degrading enzymes, cysteine cathepsins, in human dentin. This study aimed to examine the presence, source, and activity of cysteine cathepsins in human caries. Cathepsin B was detected with immunostaining. Saliva and dentin cysteine cathepsin and MMP activities on caries lesions were analyzed spectrofluorometrically. Immunostaining demonstrated stronger cathepsins B in carious than in healthy dentin. In carious dentin, cysteine cathepsin activity increased with increasing depth and age in chronic lesions, but decreased with age in active lesions. MMP activity decreased with age in both active and chronic lesions. Salivary MMP activities were higher in patients with active than chronic lesions and with increasing lesion depth, while cysteine cathepsin activities showed no differences. The results indicate that, along with MMPs, cysteine cathepsins are important, especially in active and deep caries. |
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Authors:
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F D Nascimento; C L Minciotti; S Geraldeli; M R Carrilho; D H Pashley; F R Tay; H B Nader; T Salo; L Tjäderhane; I L S Tersariol |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-01-19 |
Journal Detail:
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Title: Journal of dental research Volume: 90 ISSN: 1544-0591 ISO Abbreviation: J. Dent. Res. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-28 Completed Date: 2011-06-02 Revised Date: 2012-09-20 |
Medline Journal Info:
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Nlm Unique ID: 0354343 Medline TA: J Dent Res Country: United States |
Other Details:
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Languages: eng Pagination: 506-11 Citation Subset: D; IM |
Affiliation:
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Grupo de Estudos em Odontologia, Universidade Bandeirante de São Paulo, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Age Factors Cathepsin B / analysis Cathepsins / analysis*, antagonists & inhibitors Child Cysteine Proteases / analysis* Cysteine Proteinase Inhibitors / pharmacology Dental Caries / enzymology*, pathology Dental Pulp Exposure / enzymology Dentin / enzymology*, pathology Fluorescent Dyes / diagnostic use Glycopeptides / pharmacology Humans Leucine / analogs & derivatives, pharmacology Matrix Metalloproteinases / analysis, antagonists & inhibitors Metalloendopeptidases / antagonists & inhibitors Middle Aged Odontoblasts / enzymology Oligopeptides / diagnostic use Pepstatins / pharmacology Protease Inhibitors / pharmacology Saliva / enzymology Serine Proteinase Inhibitors / pharmacology Spectrometry, Fluorescence Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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R01 DE015306-06/DE/NIDCR NIH HHS; R01 DE015306-08/DE/NIDCR NIH HHS; R21 DE019213-02/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cysteine Proteinase Inhibitors; 0/Fluorescent Dyes; 0/Glycopeptides; 0/Oligopeptides; 0/Pepstatins; 0/Protease Inhibitors; 0/Serine Proteinase Inhibitors; 0/abz-GGFLRKHGQ-EDDnp; 36357-77-4/phosphoramidon; 39324-30-6/pepstatin; 61-90-5/Leucine; 66701-25-5/E 64; EC 3.4.-/Cathepsins; EC 3.4.-/Cysteine Proteases; EC 3.4.22.1/Cathepsin B; EC 3.4.24.-/Matrix Metalloproteinases; EC 3.4.24.-/Metalloendopeptidases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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