| Cysteamine prevents the development of lens opacity in a rat model of selenite-induced cataract. | |
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MedLine Citation:
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PMID: 22281827 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Purpose:The activation of transglutaminase 2 (TG2) by oxidative stress through TGFβ has been reported to play a crucial role in cataract formation. We investigated whether TG2 is involved in selenite-induced cataract formation in rats and whether cysteamine, a chemical inhibitor of TG2, can prevent cataract formation in this model.Methods:Intracellular TG2 activity was monitored in a human lens epithelial cell (HLE-B3) line and cultured rat lenses after treatment with selenite. Rat pups (13 days old) were injected with sodium selenite (Na(2)SeO(3); 20 µmol/kg) subcutaneously together with the intraperitoneal administration of cysteamine (30, 40, and 60 mg/kg) for 14 days. Lenses were evaluated photographically at days 7 and 14. The concentrations of malondialdehyde and glutathione in the lenses were determined.Results:In HLE-B3 cells or rat lenses, selenite induced intracellular TG activity, which was inhibited by cysteamine. In selenite-treated rats, the rate of cataract formation was significantly reduced by cysteamine (P < 0.001). The mean cataract area in the lenses of cysteamine-treated rats was smaller than that of control rats (P < 0.01). The levels of total and reduced glutathione in the lenses of cysteamine-treated rats extracted at day 14 were higher than those of control rats.Conclusion:Cysteamine suppresses cataract formation induced by selenite in rats, suggesting that cysteamine could be used as a pharmaceutical intervention to prevent or delay cataract formation. |
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Authors:
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Sang-Mok Lee; Eui Man Jeong; Jinho Jeong; Dong-Myung Shin; Hyun-Ju Lee; Hyo-Jun Kim; Jisun Lim; Jin-Haeng Lee; Sung-Yup Cho; Mee-Kum Kim; Won-Ryang Wee; Jin-Hak Lee; In-Gyu Kim |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-26 |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: - ISSN: 1552-5783 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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