Document Detail


Cystatin C is highly expressed in the human male reproductive system.
MedLine Citation:
PMID:  15223845     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cystatin C displays the strongest inhibitory activity of all cystatins toward lysosomal cysteine proteases in general and has a widespread distribution in human tissues and body fluids, including seminal plasma. The aim of this study was to investigate the distribution of cystatin C in the male reproductive system. Immunohistochemistry revealed a widespread distribution of cystatin C in normal tissues from the testis, epididymis, vas deferens, seminal vesicle, and prostate gland. Immunoreactive cystatin C was localized in basal and secretory epithelial cells, but also in neuroendocrine cells in the prostate, identified by immunostaining for chromogranin A. On adjacent tissue sections, we demonstrated local production of cystatin C utilizing nonradioactive in situ hybridization with a 201-base-long digoxigenin-labeled antisense RNA probe specific for the cystatin C transcript. Staining patterns obtained by immunohistochemistry and in situ hybridization correlated well. Enzyme-linked immunosorbent assay for quantitative analysis of cystatin C demonstrated that cystatin C was present at high concentrations in tissue homogenates from all locations investigated, compared to liver, muscle, spleen, and other general tissues. Western blotting of tissue homogenates revealed a predominant 15-kd cystatin C immunoreactive component in accordance with previous findings in other organs. Quantitative real-time polymerase chain reaction analysis to determine messenger RNA levels in whole tissue extracts showed that the cystatin C gene is highly expressed in the seminal vesicles and the prostate gland, indicating that the major amount of cystatin C in the male reproductive organs and seminal plasma is produced by cells in these 2 tissues. It is concluded that cystatin C is highly expressed and widely distributed throughout the male genital tract, suggesting that cystatin C is an important regulator for normal and pathological proteolysis in the male reproductive system.
Authors:
Thomas Jiborn; Magnus Abrahamson; Hanna Wallin; Johan Malm; Ake Lundwall; Virgil Gadaleanu; Per-Anders Abrahamsson; Anders Bjartell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of andrology     Volume:  25     ISSN:  0196-3635     ISO Abbreviation:  J. Androl.     Publication Date:    2004 Jul-Aug
Date Detail:
Created Date:  2004-06-29     Completed Date:  2005-06-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8106453     Medline TA:  J Androl     Country:  United States    
Other Details:
Languages:  eng     Pagination:  564-72     Citation Subset:  IM    
Affiliation:
Department of Urology, Malmö University Hospital, Lund University, Sweden. thomas.jiborn@skane.se
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MeSH Terms
Descriptor/Qualifier:
Cystatin C
Cystatins / genetics*
Epididymis / cytology,  physiology
Gene Expression Regulation / physiology*
Genitalia, Male / cytology,  physiology*
Humans
In Situ Hybridization
Male
Polymerase Chain Reaction / methods
Prostate / cytology,  physiology
RNA, Messenger / genetics
Seminal Vesicles / cytology,  physiology
Testis / cytology,  physiology
Vas Deferens / cytology,  physiology
Chemical
Reg. No./Substance:
0/CST3 protein, human; 0/Cystatin C; 0/Cystatins; 0/RNA, Messenger

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