Document Detail


Cyp26 enzymes are required to balance the cardiac and vascular lineages within the anterior lateral plate mesoderm.
MedLine Citation:
PMID:  24667328     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Normal heart development requires appropriate levels of retinoic acid (RA) signaling. RA levels in embryos are dampened by Cyp26 enzymes, which metabolize RA into easily degraded derivatives. Loss of Cyp26 function in humans is associated with numerous developmental syndromes that include cardiovascular defects. Although previous studies have shown that Cyp26-deficient vertebrate models also have cardiovascular defects, the mechanisms underlying these defects are not understood. Here, we found that in zebrafish, two Cyp26 enzymes, Cyp26a1 and Cyp26c1, are expressed in the anterior lateral plate mesoderm (ALPM) and predominantly overlap with vascular progenitors (VPs). Although singular knockdown of Cyp26a1 or Cyp26c1 does not overtly affect cardiovascular development, double Cyp26a1 and Cyp26c1 (referred to here as Cyp26)-deficient embryos have increased atrial cells and reduced cranial vasculature cells. Examining the ALPM using lineage tracing indicated that in Cyp26-deficient embryos the myocardial progenitor field contains excess atrial progenitors and is shifted anteriorly into a region that normally solely gives rise to VPs. Although Cyp26 expression partially overlaps with VPs in the ALPM, we found that Cyp26 enzymes largely act cell non-autonomously to promote appropriate cardiovascular development. Our results suggest that localized expression of Cyp26 enzymes cell non-autonomously defines the boundaries between the cardiac and VP fields within the ALPM through regulating RA levels, which ensures a proper balance of myocardial and endothelial lineages. Our study provides novel insight into the earliest consequences of Cyp26 deficiency that underlie cardiovascular malformations in vertebrate embryos.
Authors:
Ariel B Rydeen; Joshua S Waxman
Related Documents :
16019308 - Peptic digestibility of raw and heat-coagulated hen's egg white proteins at acidic ph r...
9169008 - Characterization of linamarase of latex and its localization in petioles in cassava.
6773658 - The effects of n-substitution of chitosan and the physical form of the products on the ...
20380848 - Chemical analysis of hericium erinaceum polysaccharides and effect of the polysaccharid...
4658988 - Aspartate transcarbamoylase from phaseolus aureus. partial purification and properties.
7642698 - Wortmannin causes mistargeting of procathepsin d. evidence for the involvement of a pho...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-3-25
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  -     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2014 Mar 
Date Detail:
Created Date:  2014-3-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Type I TARPs promote dendritic growth of early postnatal neocortical pyramidal cells in organotypic ...
Next Document:  Degradation of 4-chloro-3-nitrophenol via a novel intermediate, 4-chlororesorcinol by Pseudomonas sp...