Document Detail


Cynomorium songaricum extracts functionally modulate transporters of gamma-aminobutyric acid and monoamine.
MedLine Citation:
PMID:  20084455     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cynomorium songaricum Rupr. (SY) is a central nervous system-oriented herb material that has actions of anti-dementia, anti-epilepsy, and anti-stress. It is unclear whether SY would be biologically active in functionally regulating neurotransmitter transporters. Here, we assessed these potential actions using Chinese hamster ovary cells expressing gamma-aminobutyric acid (GABA) transporter (GAT-1), dopamine transporter (DAT), norepinephrine transporter (NET), or serotonin transporter (SERT) (i.e. G1, D8, N1, or S6 cells, respectively). It was shown that SY extracts, such as SYw, SYa, SYp, SYc, SYe, and SYb (SY water, ethanol, petroleum ether, chloroform, ethyl acetate, and n-butyl alcohol extract, respectively) increased dopamine/norepinephrine (DA/NE) uptake by corresponding D8/N1 cells and decreased gamma-aminobutyric acid/serotoin (GABA/5HT) uptake by corresponding G1/S6 cells; wherein, the potency or efficacy of SYc for up-regulating DA/NE uptake and that of SYb for inhibiting GABA/5HT uptake were relatively stronger. Additionally, GABA/5H-uptake inhibition by SY extracts were also seen in cortical synaptosomes, and DA/NE-uptake enhancement by SYc was dependent on the activity of DAT and NET. Thus, SY extracts especially SYc and SYb are novel neurotransmitter-transporter modulators functioning as DAT/NET activators and/or GAT-1/SERT inhibitors.
Authors:
Gang Zhao; Jie Wang; Guo-Wei Qin; Li-He Guo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurochemical research     Volume:  35     ISSN:  1573-6903     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-18     Completed Date:  2010-08-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  666-76     Citation Subset:  IM    
Affiliation:
Cell Star Bio-Technologies Co., Limited, Shanghai, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / drug effects,  metabolism
CHO Cells
Cricetinae
Cricetulus
Cynomorium / chemistry*
Dopamine Plasma Membrane Transport Proteins / metabolism*
GABA Plasma Membrane Transport Proteins / metabolism*
Norepinephrine Plasma Membrane Transport Proteins / metabolism*
Plant Extracts / pharmacology*
Serotonin / metabolism
Serotonin Plasma Membrane Transport Proteins / metabolism*
Synaptosomes / drug effects,  metabolism
gamma-Aminobutyric Acid / metabolism
Chemical
Reg. No./Substance:
0/Dopamine Plasma Membrane Transport Proteins; 0/GABA Plasma Membrane Transport Proteins; 0/Norepinephrine Plasma Membrane Transport Proteins; 0/Plant Extracts; 0/Serotonin Plasma Membrane Transport Proteins; 50-67-9/Serotonin; 56-12-2/gamma-Aminobutyric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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