Document Detail


Cylindrical compact thermal-cycling device for continuous-flow polymerase chain reaction.
MedLine Citation:
PMID:  14588047     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
A compact, thermal-cycling device for high-throughput continuous-flow polymerase chain reaction (PCR) has been developed, which consists of a flow-through capillary and a cylindrical heating-block assembly. A 3.5-m-long fused-silica capillary coils helically, with 33 turns, up around the 30-mm-diameter assembly of three equally divided thermostating copper blocks for melting, annealing, and extension. An injected PCR mixture undergoes one cycle of PCR each turn. A continuous-flow PCR of one sample and also a segmented-flow PCR of four different samples have been successfully demonstrated. The present device can easily evolve into a parallel-processing, multistation compact device and be modified to have real-time PCR capability. This solid-based compact PCR device, therefore, has a potentiality to be the format of choice when developed for a portable system.
Authors:
Nokyoung Park; Suhyeon Kim; Jong Hoon Hahn
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical chemistry     Volume:  75     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-10-31     Completed Date:  2004-06-08     Revised Date:  2004-06-10    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6029-33     Citation Subset:  -    
Affiliation:
National Research Laboratory for Advanced Biotechnology and Biomedical Microinstrumentation, Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology, San 31 Hyoja-Dong, Pohang 790-784, South Korea.
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Erratum In:
Anal Chem. 2004 Feb 15;76(4):1211

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