Document Detail


Cyclopropanation of α-mycolic acids is not required for cording in Mycobacterium brumae and Mycobacterium fallax.
MedLine Citation:
PMID:  22493302     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The capacity to form microscopic cords (cording) of Mycobacterium species has been related to their virulence. The compounds responsible for cording are unknown, but a recent study has shown that cording could be related to the fine structure of α-mycolic acids. This investigation attributes the need for a proximal cyclopropane in α-mycolic acids for cording in Mycobacterium tuberculosis and Mycobacterium bovis BCG and proposes cyclopropanases as good targets for new chemotherapeutic agents. As other Mycobacterium species form microscopic cords in addition to M. tuberculosis and M. bovis, it would be of major interest to know whether the relationship between proximal cyclopropanation of α-mycolic acids and cording could be extended to non-tuberculous mycobacteria. In this study, we have examined the correlation between the cording and cyclopropanation of α-mycolic acids in two species, Mycobacterium brumae and Mycobacterium fallax. Scanning electron microscopy (SEM) images showed, for the first time, the fine structure of microscopic cords of M. brumae and M. fallax, confirming that these two species form true cords. Furthermore, nuclear magnetic resonance spectroscopy (NMR) analysis, performed on the same cording cultures, corroborate the absence of cyclopropane rings in their α-mycolic acids. Therefore, we can conclude that the correlation between cording and cyclopropanation of α-mycolic acids cannot be extended to all mycobacteria. As M. brumae and M. fallax are rapid growers and have a simple pattern of mycolic acids (only α-unsaturated-mycolic acids), we propose these two species as suitable models for the study of the role of mycolic acids in cording.
Authors:
Cecilia Brambilla; Alejandro Sánchez-Chardi; Míriam Pérez-Trujillo; Esther Julián; Marina Luquin
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-5
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  -     ISSN:  1465-2080     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Universitat Autònoma de Barcelona.
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