Document Detail

Cyclooxygenases in human and mouse skin and cultured human keratinocytes: association of COX-2 expression with human keratinocyte differentiation.
MedLine Citation:
PMID:  8612694     Owner:  NLM     Status:  MEDLINE    
Epidermal expression of the two isoforms of the prostaglandin H-generating cyclooxygenase (COX-1 and COX-2) was evaluated both by immunohistochemistry performed on human and mouse skin biopsy sections and by Western blotting of protein extracts from cultured human neonatal foreskin keratinocytes. In normal human skin, COX-1 immunostaining is observed throughout the epidermis whereas COX-2 immunostaining increases in the more differentiated, suprabasilar keratinocytes. Basal cell carcinomas express little if any COX-1 or COX-2 immunostaining whereas both isozymes are strongly expressed in squamous cell carcinomas deriving from a more differentiated layer of the epidermis. In human keratinocyte cultures, raising the extracellular calcium concentration, a recognized stimulus for keratinocyte differentiation, leads to an increased expression of both COX-2 protein and mRNA; expression of COX-1 protein, however, shows no significant alteration in response to calcium. Because of a recent report that failed to show COX-2 in normal mouse epidermis, we also looked for COX-1 and COX-2 immunostaining in sections of normal and acetone-treated mouse skin. In agreement with a previous report, some COX-1, but no COX-2, immunostaining is seen in normal murine epidermis. However, following acetone treatment, there is a marked increase in COX-1 expression as well as the appearance of significant COX-2 immunostaining in the basal layer. These data suggest that in human epidermis as well as in human keratinocyte cultures, the expression of COX-2 occurs as a part of normal keratinocyte differentiation whereas in murine epidermis, its constitutive expression is absent, but inducible as previously published.
J Leong; M Hughes-Fulford; N Rakhlin; A Habib; J Maclouf; M E Goldyne
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  224     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-06-05     Completed Date:  1996-06-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  79-87     Citation Subset:  IM; S    
Veterans Affairs Medical Center, San Francisco 94121, USA.
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MeSH Terms
Blotting, Western
Cell Differentiation
Cells, Cultured
Cyclooxygenase 1
Cyclooxygenase 2
Isoenzymes / isolation & purification
Keratinocytes / enzymology*
Membrane Proteins
Prostaglandin-Endoperoxide Synthases / isolation & purification*
RNA, Messenger / analysis
Skin / enzymology*
Transcription, Genetic
Reg. No./Substance:
0/Isoenzymes; 0/Membrane Proteins; 0/RNA, Messenger; EC 1; EC 2; EC protein, human; EC protein, human; EC Synthases; EC protein, mouse
M Hughes-Fulford / Dept Veterans' Affairs, San Francisco CA

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