| Cyclooxygenase inhibitors enhance cell growth in an osteoblastic cell line, MC3T3-E1. | |
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MedLine Citation:
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PMID: 2510469 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To elucidate the significance of endogenous prostaglandin E2 (PGE2) in osteoblastic cell function, we studied the effects of cyclooxygenase inhibitors on cell growth and alkaline phosphatase (ALP) activity in MC3T3-E1 cells. UMR-106 cells were also used as references in our experiments. MC3T3-E1 cells, cultured in alpha-minimal essential medium containing 10% fetal bovine serum, were shown to produce PGE2, which was markedly suppressed in the presence of indomethacin. Addition of indomethacin resulted in an increase in DNA content and [3H]thymidine incorporation. A similar growth stimulatory effect was observed when structurally different cyclooxygenase inhibitors, that is, acetyl salicylic acid (ASA), flurbiprofen, and piroxicam, were added. These cyclooxygenase inhibitors, however, differed in their effects on ALP activity. Indomethacin and ASA enhanced ALP activity, whereas flurbiprofen and piroxicam suppressed it. We then examined the effects of exogenous addition of PGE2. Although exogenous PGE2 at 6 x 10(-6) M slightly stimulated cell growth, it inhibited cell growth at 6 x 10(-8) M and 6 x 10(-7) M. ALP activity was reduced in a dose-dependent fashion by exogenous PGE2. These results suggest that PGE2 produced by MC3T3-E1 may be suppressing cell proliferation and that cyclooxygenase inhibitors, per se, may stimulate cell growth by inhibiting endogenous PGE2 production in MC3T3-E1 cells. UMR-106 cells also produced PGE2, although less than MC3T3-E1 cells. In UMR-106 cells, the cyclooxygenase inhibitors did not influence DNA content or ALP activity as distinctly as in MC3T3-E1 cells.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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A Fujimori; M Tsutsumi; M Fukase; T Fujita |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research Volume: 4 ISSN: 0884-0431 ISO Abbreviation: J. Bone Miner. Res. Publication Date: 1989 Oct |
Date Detail:
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Created Date: 1989-12-08 Completed Date: 1989-12-08 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8610640 Medline TA: J Bone Miner Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 697-704 Citation Subset: IM |
Affiliation:
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Department of Medicine, Kobe University School of Medicine. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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metabolism Animals Anti-Inflammatory Agents, Non-Steroidal / pharmacology* Cell Division / drug effects Cell Line Cyclooxygenase Inhibitors DNA / analysis, biosynthesis Dinoprostone / metabolism Mice Osteoblasts / cytology, drug effects* Rats Thymidine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents, Non-Steroidal; 0/Cyclooxygenase Inhibitors; 363-24-6/Dinoprostone; 50-89-5/Thymidine; 9007-49-2/DNA; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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