| Cyclooxygenase-catalyzed formation of 9-hydroxylinoleic acid by guinea pig alveolar macrophages under non-stimulated conditions. | |
| | |
MedLine Citation:
|
PMID: 3098580 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Data are presented on the formation of a linoleic acid oxygenation product under basal conditions by guinea pig alveolar macrophages (AM). Under non-stimulated conditions 20 X 10(6) AM released about 1 nmol 9-hydroxylinoleic acid (9-HODE) during a 15 min incubation. Furthermore, AM released more than 2 nmol 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT), a byproduct of the formation of thromboxane A2. 9-HODE and HHT were the most prominent products formed under the incubation conditions used. These products were formed via a cyclooxygenase-catalyzed reaction, since their formation was inhibited by 1 microM indomethacin. Thus, under basal conditions AM possess substantial cyclooxygenase activity. The biological significance of 9-HODE and related substances is discussed. |
| | |
Authors:
|
F Engels; H Willems; F P Nijkamp |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: FEBS letters Volume: 209 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1986 Dec |
Date Detail:
|
Created Date: 1987-02-17 Completed Date: 1987-02-17 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 249-53 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chromatography, High Pressure Liquid Fatty Acids / isolation & purification Guinea Pigs Indomethacin / pharmacology Linoleic Acids / biosynthesis* Linoleic Acids, Conjugated* Macrophages / drug effects, enzymology* Male Prostaglandin-Endoperoxide Synthases / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Fatty Acids; 0/Linoleic Acids; 0/Linoleic Acids, Conjugated; 15514-85-9/9-hydroxy-10,12-octadecadienoic acid; 53-86-1/Indomethacin; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Lipid peroxidation in regenerating rat liver.
Next Document: Origins of stereospecificity in DNA damage by anti-benzo[a]pyrene diol-epoxides. A molecular modelli...