Document Detail

Cyclooxygenase-catalyzed formation of 9-hydroxylinoleic acid by guinea pig alveolar macrophages under non-stimulated conditions.
MedLine Citation:
PMID:  3098580     Owner:  NLM     Status:  MEDLINE    
Data are presented on the formation of a linoleic acid oxygenation product under basal conditions by guinea pig alveolar macrophages (AM). Under non-stimulated conditions 20 X 10(6) AM released about 1 nmol 9-hydroxylinoleic acid (9-HODE) during a 15 min incubation. Furthermore, AM released more than 2 nmol 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT), a byproduct of the formation of thromboxane A2. 9-HODE and HHT were the most prominent products formed under the incubation conditions used. These products were formed via a cyclooxygenase-catalyzed reaction, since their formation was inhibited by 1 microM indomethacin. Thus, under basal conditions AM possess substantial cyclooxygenase activity. The biological significance of 9-HODE and related substances is discussed.
F Engels; H Willems; F P Nijkamp
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  209     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1986 Dec 
Date Detail:
Created Date:  1987-02-17     Completed Date:  1987-02-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  249-53     Citation Subset:  IM    
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MeSH Terms
Chromatography, High Pressure Liquid
Fatty Acids / isolation & purification
Guinea Pigs
Indomethacin / pharmacology
Linoleic Acids / biosynthesis*
Linoleic Acids, Conjugated*
Macrophages / drug effects,  enzymology*
Prostaglandin-Endoperoxide Synthases / metabolism*
Reg. No./Substance:
0/Fatty Acids; 0/Linoleic Acids; 0/Linoleic Acids, Conjugated; 15514-85-9/9-hydroxy-10,12-octadecadienoic acid; 53-86-1/Indomethacin; EC Synthases

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