Document Detail


Cyclooxygenase-2 and inducible nitric oxide synthase gene polymorphisms and risk of reflux esophagitis, Barrett's esophagus, and esophageal adenocarcinoma.
MedLine Citation:
PMID:  18349295     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The incidence of esophageal adenocarcinoma has increased in recent years, and Barrett's esophagus is a recognized risk factor. Gastroesophageal reflux of acid and/or bile is linked to these conditions and to reflux esophagitis. Inflammatory disorders can lead to carcinogenesis through activation of "prosurvival genes," including cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Increased expression of these enzymes has been found in esophageal adenocarcinoma, Barrett's esophagus, and reflux esophagitis. Polymorphic variants in COX-2 and iNOS genes may be modifiers of risk of these conditions. In a population-based case-control study, we examined associations of the COX-2 8473 T>C and iNOS Ser(608) Leu (C>T) polymorphisms with risk of esophageal adenocarcinoma, Barrett's esophagus, and reflux esophagitis. Genomic DNA was extracted from blood samples collected from cases of esophageal adenocarcinoma (n = 210), Barrett's esophagus (n = 212), and reflux esophagitis (n = 230) and normal population controls frequency matched for age and sex (n = 248). Polymorphisms were genotyped using TaqMan allelic discrimination assays. Odds ratios and 95% confidence intervals were obtained from logistic regression models adjusted for potential confounding factors. The presence of at least one COX-2 8473 C allele was associated with a significantly increased risk of esophageal adenocarcinoma (adjusted odds ratio, 1.58; 95% confidence interval, 1.04-2.40). There was no significant association between this polymorphism and risk of Barrett's esophagus or reflux esophagitis or between the iNOS Ser 608 Leu polymorphism and risk of these esophageal conditions. Our study suggests that the COX-2 8473 C allele is a potential genetic marker for susceptibility to esophageal adenocarcinoma.
Authors:
Heather R Ferguson; Christopher P Wild; Lesley A Anderson; Seamus J Murphy; Brian T Johnston; Liam J Murray; R G Peter Watson; Jim McGuigan; John V Reynolds; Laura J Hardie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology     Volume:  17     ISSN:  1055-9965     ISO Abbreviation:  Cancer Epidemiol. Biomarkers Prev.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-03-19     Completed Date:  2008-06-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9200608     Medline TA:  Cancer Epidemiol Biomarkers Prev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  727-31     Citation Subset:  IM    
Affiliation:
Division of Gastroenterology, Belfast City Hospital, Belfast, United Kingdom. hrferguson@doctors.net.uk
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / enzymology*,  genetics*
Alleles
Barrett Esophagus / enzymology*,  genetics*
Case-Control Studies
Cyclooxygenase 2 / genetics*
Esophageal Neoplasms / enzymology*,  genetics*
Esophagitis, Peptic / enzymology*,  genetics*
Female
Genetic Markers
Genetic Variation
Humans
Logistic Models
Male
Middle Aged
Nitric Oxide Synthase Type II / genetics*
Polymorphism, Genetic*
Chemical
Reg. No./Substance:
0/Genetic Markers; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.99.1/Cyclooxygenase 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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