Document Detail


Cyclooxygenase-2 expression in primary human colorectal cancers and bone marrow micrometastases.
MedLine Citation:
PMID:  15248379     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Both the expressions of the inducible form of cyclooxygenase-2 and the presence of bone marrow micrometastases are poor prognostic markers in patients with colorectal carcinoma. AIMS: As cyclooxygenase-2 expression in these tumours is associated with increased metastatic potential in vitro, our objectives were to determine the relationship between cyclooxygenase-2 and haematogenous spread to bone marrow. PATIENTS AND METHODS: Thirty-two patients with resection of colorectal carcinoma were evaluated (median age: 69.5 years). Bone marrow was obtained from all patients from both iliac crests before manipulation of the primary tumour. The tumours were of varying stages at diagnosis (5 Dukes' A, 14 Dukes' B, 11 Dukes' C and 2 Dukes' D). Tumour sections were stained for cyclooxygenase-2 using the avidin-biotin immunohistochemical technique. Extent of staining was graded depending on the percentage of epithelial cells staining positive for cyclooxygenase-2. Micrometastases were detected by staining contaminant cytokeratin-18 positive cells in the bone marrow aspirates by either immunohistochemical (ARAAP) or immunological (flow cytometry) methods. Fisher's exact probability test was used to calculate statistical significance. RESULTS: Cyclooxygenase-2 expression in the primary tumour was detected in 72% of the patients. Twelve (38%) patients had bone marrow micrometastases detected by either immunohistochemistry or flow cytometry. Of the 12 patients who had bone marrow micrometastases, 8 tumours demonstrated increased expression of cyclooxygenase-2 protein (66.6%). In contrast, 9 out of the 20 (45%) patients in whom micrometastases were not detected expressed increased levels of cyclooxygenase-2 (P = 0.29). When dividing the patients into subgroups of localised (Dukes' A and B) versus disseminated (Dukes' C and D) disease, there was no further association between cyclooxygenase-2 expression and bone marrow micrometastases (P = 0.179 and 1.0). CONCLUSION: In this pilot study, there was no association between cyclooxygenase-2 expression and bone marrow micrometastases in patients with otherwise localised or disseminated disease.
Authors:
K M Sheehan; R A Cahill; G McGreal; C Steele; M F Byrne; W O Kirwan; E W Kay; D J Fitzgerald; H P Redmond; F E Murray
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver     Volume:  36     ISSN:  1590-8658     ISO Abbreviation:  Dig Liver Dis     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-07-13     Completed Date:  2004-11-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100958385     Medline TA:  Dig Liver Dis     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  392-7     Citation Subset:  IM    
Affiliation:
Department of Pathology, Beaumont Hospital and Royal College of Surgeons, Beaumont Road, Dublin 9, Ireland. katherinesheehan@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Bone Marrow Neoplasms / enzymology*,  pathology,  secondary*
Colorectal Neoplasms / enzymology*,  pathology
Cyclooxygenase 2
Epithelial Cells / enzymology
Female
Flow Cytometry
Humans
Immunoenzyme Techniques
Isoenzymes / biosynthesis*
Male
Membrane Proteins
Middle Aged
Peroxidases / biosynthesis
Pilot Projects
Prostaglandin-Endoperoxide Synthases / biosynthesis*
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/Membrane Proteins; EC 1.11.1.-/Peroxidases; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases

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