Document Detail

Cycloheximide-induced phase shifting of circadian clock of Neurospora.
MedLine Citation:
PMID:  6454353     Owner:  NLM     Status:  MEDLINE    
Cycloheximide (CHX), an inhibitor of cytosolic (80S) protein synthesis in eucaryotes, causes phase shifts of the circadian clock of Neurospora crassa when administered as 4-h pulses to cultures in liquid medium. Differential effects of the pulses at different phases of the circadian cycle were observed and plotted as a phase-response curve (PRC). Nearly all phase shifts observed were phase advances, with maximum sensitivity in the middle of the subjective day. Inhibition of protein synthesis by CHX was the same at both phases of the cycle. The PRC was the same at 20 and 25 degrees C. Dose-response curves for the effects of CHX on phase shifting and inhibition of protein synthesis were determined and showed a striking parallel in the responses of these two phenomena to CHX. These results support the view that synthesis of one or more proteins at specific phases of the circadian cycle is necessary for the normal operation of the circadian clock of Neurospora.
H Nakashima; J Perlman; J F Feldman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  241     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1981 Jul 
Date Detail:
Created Date:  1981-08-27     Completed Date:  1981-08-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R31-5     Citation Subset:  IM    
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MeSH Terms
Biological Transport / drug effects
Circadian Rhythm / drug effects*
Cycloheximide / pharmacology*
Dose-Response Relationship, Drug
Fungal Proteins / biosynthesis
Leucine / metabolism
Neurospora / growth & development*
Neurospora crassa / drug effects,  growth & development*
Grant Support
Reg. No./Substance:
0/Fungal Proteins; 61-90-5/Leucine; 66-81-9/Cycloheximide

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