| Cyclin A is required at two points in the human cell cycle. | |
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MedLine Citation:
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PMID: 1312467 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cyclins play a fundamental role in regulating cell cycle events in all eukaryotic cells. The human cyclin A gene was identified as the site of integration of hepatitis B virus in a hepatocarcinoma cell line; in addition, cyclin A is associated with the E2F transcription factor in a complex which is dissociated by the E1A oncogene product. Such findings suggest that cyclin A is a target for oncogenic signals. We have now found that DNA synthesis and entry into mitosis are inhibited in human cells microinjected with anti-cyclin A antibodies at distinct times. Cyclin A binds both cdk2 and cdc2, giving two distinct cyclin A kinase activities, one appearing in S phase, the other in G2. These results suggest that cyclin A defines novel control points of the human cell cycle. |
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Authors:
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M Pagano; R Pepperkok; F Verde; W Ansorge; G Draetta |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The EMBO journal Volume: 11 ISSN: 0261-4189 ISO Abbreviation: EMBO J. Publication Date: 1992 Mar |
Date Detail:
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Created Date: 1992-04-21 Completed Date: 1992-04-21 Revised Date: 2010-09-07 |
Medline Journal Info:
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Nlm Unique ID: 8208664 Medline TA: EMBO J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 961-71 Citation Subset: IM |
Affiliation:
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Differentiation, Programme European Molecular Biology Laboratory, Heidelberg, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenovirus Early Proteins Amino Acid Sequence Blotting, Western CDC2 Protein Kinase / metabolism CDC2-CDC28 Kinases* Carrier Proteins* Cell Cycle* Cell Cycle Proteins* Cyclin-Dependent Kinase 2 Cyclin-Dependent Kinases* Cyclins / genetics, immunology, metabolism* DNA Replication DNA-Binding Proteins* E2F Transcription Factors Fluorescent Antibody Technique Gene Expression Hela Cells Humans Immunohistochemistry Kinetics Microinjections Molecular Sequence Data Oncogene Proteins, Viral / metabolism Phosphotransferases / metabolism Plasmids Protein Kinases / metabolism Protein-Serine-Threonine Kinases* Recombinant Proteins / genetics, metabolism Retinoblastoma-Binding Protein 1 Transcription Factor DP1 Transcription Factors / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Adenovirus Early Proteins; 0/Carrier Proteins; 0/Cell Cycle Proteins; 0/Cyclins; 0/DNA-Binding Proteins; 0/E2F Transcription Factors; 0/Oncogene Proteins, Viral; 0/Recombinant Proteins; 0/Retinoblastoma-Binding Protein 1; 0/Transcription Factor DP1; 0/Transcription Factors; EC 2.7.-/Phosphotransferases; EC 2.7.-/Protein Kinases; EC 2.7.1.37/CDK2 protein, human; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.22/CDC2 Protein Kinase; EC 2.7.11.22/CDC2-CDC28 Kinases; EC 2.7.11.22/Cyclin-Dependent Kinase 2; EC 2.7.11.22/Cyclin-Dependent Kinases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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