Document Detail


Cyclin A, cyclin B and stringlike are regulated separately in cell cycle arrested trochoblasts of Patella vulgata embryos.
MedLine Citation:
PMID:  9510547     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Trochoblasts are the first cells to differentiate during the development of spiralian embryos. Differentiation is accompanied by a cell division arrest. In embryos of the limpet Patella vulgata, the participation of cell cycle-regulating factors in trochoblast arrest was analysed as a first step to unravel its cause. We determined the cell cycle phase in which the trochoblasts are arrested by analysing the subcellular locations of mitotic cyclins. The results show that the trochoblasts are most likely arrested in the G2 phase. This was supported by measurement of the DNA content in trochoblast nuclei after the last division. Trochoblasts complete their final division at the sixth mitotic cycle. This mitotic cycle resembles the first postblastoderm cell cycle of Drosophila, in which mitotic activity is controlled by expression of the string gene. As failure of string expression results in cell cycle arrest in the G2 phase, negative regulation of a Patella string homolog could be responsible for trochoblast arrest. Although Stl messengers disappeared from trochoblasts during their final division, expression was observed again 20 min later. Messengers remained present in all trochoblasts at low levels during further development. Thus, expression of the stringlike gene allows the cell cycle arrest of these cells, whereas in Drosophila cells arrested in division lack string messengers.
Authors:
A van der Kooij; C P Veraart; A E van Loon
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Development genes and evolution     Volume:  207     ISSN:  0949-944X     ISO Abbreviation:  Dev. Genes Evol.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-04-21     Completed Date:  1998-04-21     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9613264     Medline TA:  Dev Genes Evol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  524-34     Citation Subset:  IM    
Affiliation:
Department of Experimental Zoology, Universiteit Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle
Cell Cycle Proteins / genetics,  physiology*
Cell Nucleus / metabolism
Cyclin A / genetics,  physiology*
Cyclin B / genetics,  physiology*
DNA / biosynthesis
Drosophila Proteins
G2 Phase
Mitosis
Mollusca / embryology*
RNA, Messenger / metabolism
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/CycB protein, Drosophila; 0/Cyclin A; 0/Cyclin B; 0/Drosophila Proteins; 0/RNA, Messenger; 0/stringlike protein, Patella vulgata; 9007-49-2/DNA

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