| Cyclin D1 overexpression permits the reproducible detection of senescent human vascular smooth muscle cells. | |
| | |
MedLine Citation:
|
PMID: 18056951 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The senescence of mitotic cells is hypothesized to play a causal role in organismal aging. Cultures of normal human cells become senescent in vitro as a result of a continuous decline in the mitotic fraction from cell turnover. However, one potential barrier to the evaluation of the frequency and distribution of senescent cells in tissues is the absence of a panel of robust markers for the senescent state. In parallel with an analysis of the growth kinetics of human vascular smooth muscle cells, we have undertaken transcriptomic comparisons of early- and late-passage cultures of human vascular smooth muscle cells to identify potential markers that can distinguish between senescent and growth-competent cells. A wide range of genes are upregulated at senescence in human vascular smooth muscle cells. In particular, we have identified a 12-fold upregulation of expression in the cyclin D1 message, which is reflected in a concomitant upregulation at the protein level. Quantitative cytochemical analysis of senescent and growing vascular smooth muscle cells indicates that cyclin D1 reactivity is a considerably better marker of replicative senescence than senescence-associated beta-galactosidase activity. We have applied this new marker (in combination with Ki67, COMET, and TUNEL staining) to the study of human vascular smooth muscle cells treated with resveratrol, a putative anti-aging molecule known to have significant effects on cell growth. |
| | |
Authors:
|
Dominick G A Burton; Angela N Sheerin; Elizabeth L Ostler; Kaye Smith; Peter J Giles; Jill Lowe; William Rhys-Williams; David G Kipling; Richard G A Faragher |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Annals of the New York Academy of Sciences Volume: 1119 ISSN: 0077-8923 ISO Abbreviation: Ann. N. Y. Acad. Sci. Publication Date: 2007 Nov |
Date Detail:
|
Created Date: 2007-12-06 Completed Date: 2008-03-04 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 7506858 Medline TA: Ann N Y Acad Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 20-31 Citation Subset: IM |
Affiliation:
|
School of Pharmacy and Biomolecular Sciences, Cockcroft Building, University of Brighton, Brighton, East Sussex, BN2 4GJ, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aging
/
physiology Biological Markers / metabolism Cell Aging / physiology* Cells, Cultured Comet Assay Cyclin D Cyclins / biosynthesis* Humans In Situ Nick-End Labeling Ki-67 Antigen / biosynthesis Mitosis / physiology* Muscle, Smooth, Vascular / cytology, metabolism* Myocytes, Smooth Muscle / cytology, metabolism* RNA, Messenger / biosynthesis Transcription, Genetic / physiology* Up-Regulation / physiology beta-Galactosidase / biosynthesis |
| Chemical | |
Reg. No./Substance:
|
0/Biological Markers; 0/Cyclin D; 0/Cyclins; 0/Ki-67 Antigen; 0/RNA, Messenger; EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Exposure of human diploid fibroblasts to hypoxia extends proliferative life span.
Next Document: Morphological changes associated with aging: age spots and the microinflammatory model of skin aging...