Document Detail


Cyclin A1 protein shows haplo-insufficiency for normal fertility in male mice.
MedLine Citation:
PMID:  15047941     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In higher eukaryotes, the cyclins constitute a family of proteins involved in progression through the cell cycle. The cyclin A1 gene (Ccna1) is expressed during meiosis and is required for spermatogenesis. Targeted disruption of the Ccna1 gene with a LacZ reporter gene has allowed us to study the expression pattern of this gene in more detail. We have confirmed expression in mouse pre-meiotic spermatocytes and also detected expression in the accessory olfactory bulb, hippocampus and amygdala of the adult brain. We have also found that the amount of cyclin A1 protein influences the fertility of male mice and its action is modulated by genetic background. On an outbred genetic background (129S6/SvEv x MF1), Ccna1 (tm1Col) -/- animals are sterile due to spermatogenic arrest prior to the first meiotic division while Ccna1 (tm1Col) +/- mice show reduced sperm production and fertility. This is even more pronounced on an inbred genetic background (129S6/SvEv) where Ccna1 (tm1Col) +/- male mice are sterile due to a severe reduction in the total number of sperm.
Authors:
Tiffany van der Meer; W-Y Iris Chan; Luis S Palazon; Conrad Nieduszynski; Martin Murphy; Joelle Sobczak-Thépot; Mark Carrington; William H Colledge
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  127     ISSN:  1470-1626     ISO Abbreviation:  Reproduction     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-29     Completed Date:  2004-06-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  503-11     Citation Subset:  IM    
Affiliation:
Department of Physiology, University of Cambridge, Cambridge CB2 3EG, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain Chemistry
Cyclin A / analysis,  physiology*
Cyclin A1
Fertility / physiology*
Haploidy
Homozygote
Male
Mice
Mice, Inbred C57BL
Oligospermia / metabolism
Organ Size / genetics
Seminiferous Tubules / chemistry
Sperm Count
Testis / anatomy & histology
Chemical
Reg. No./Substance:
0/Ccna1 protein, mouse; 0/Cyclin A; 0/Cyclin A1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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