Document Detail

Cyclical changes in sperm volume during in vitro incubation under capacitating conditions: a novel boar semen characteristic.
MedLine Citation:
PMID:  10864792     Owner:  NLM     Status:  MEDLINE    
The osmotic reactivity of boar spermatozoa during incubation in vitro was studied using a hypo-osmotic swelling test in conjunction with electronic measurement of cell volume. Sperm populations showed fluctuations in both iso-osmotic cell volume and hypo-osmotic volume response that fitted mathematical models for periodicity. Significant differences of frequency and amplitude were observed during sperm incubation under capacitating conditions as compared with those under non-capacitating conditions. In addition, different boars showed specific differences in their fluctuation characteristics under capacitating conditions. During incubation under capacitating conditions, a decrease in osmotic reactivity was observed that correlated with a decrease in motility, while the absolute value of the earliest maximum of the osmotic-induced response correlated with an increase in the proportion of discharged acrosomes. The time course of the cyclical behaviour of osmotic reactivity may be a useful parameter for assessing boar sperm response to capacitating conditions.
A M Petrounkina; R A Harrison; R Petzoldt; K F Weitze; E Töpfer-Petersen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  118     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  2000 Mar 
Date Detail:
Created Date:  2000-07-14     Completed Date:  2000-07-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  283-93     Citation Subset:  IM    
Institute for Reproductive Medicine, School of Veterinary Medicine Hannover, Buenteweg 15, 30559 Hannover, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Size
Cells, Cultured
Semen Preservation / methods*,  veterinary
Sperm Capacitation*
Spermatozoa / cytology*,  drug effects,  metabolism
Swine / physiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Optimal physicochemical conditions for the manipulation and short-term preservation of koala (Phasco...
Next Document:  Impact of photoperiodic exposures during late gestation and lactation periods on the pineal and repr...