Document Detail


Cyclic variations in the permeability of the cell wall of Saccharomyces cerevisiae.
MedLine Citation:
PMID:  1722597     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To study cell-cycle-related variations in wall permeability of Saccharomyces cerevisiae, two approaches were used. First, an asynchronous culture was fractionated by centrifugal elutriation into subpopulations containing cells of increasing size. The subpopulations represented different stages of the cell cycle as judged by light microscopy. Cell wall porosity increased when these subpopulations became enriched with budded cells. Secondly, synchronous cultures were obtained by releasing MATa cells from alpha-factor induced G1-arrest. These cultures grew synchronously for at least two generations. The cell wall porosity increased sharply in these cultures, shortly before buds became visible and was maximal during the initial stages of bud growth. It decreased in cells which had completed nuclear migration and before abscission of the bud had occurred. The porosity reached its lowest value during abscission and in unbudded cells. We examined the incorporation of mannoproteins into the wall during the cell cycle. SDS-extractable mannoproteins were incorporated continuously. However, the incorporation of glucanase-extractable mannoproteins, which are known to affect cell wall porosity, showed cyclic oscillations and reached its maximum after nuclear migration. This coincided with a rapid decrease in cell wall porosity, indicating that glucanase-extractable mannoproteins might contribute to this decrease.
Authors:
J G De Nobel; F M Klis; A Ram; H Van Unen; J Priem; T Munnik; H Van Den Ende
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Yeast (Chichester, England)     Volume:  7     ISSN:  0749-503X     ISO Abbreviation:  Yeast     Publication Date:    1991 Aug-Sep
Date Detail:
Created Date:  1992-02-18     Completed Date:  1992-02-18     Revised Date:  2002-11-01    
Medline Journal Info:
Nlm Unique ID:  8607637     Medline TA:  Yeast     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  589-98     Citation Subset:  IM    
Affiliation:
University of Amsterdam, Molecular Cell Biology, Biotechnology Center, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Benzenesulfonates
Cell Cycle*
Cell Membrane Permeability*
Cell Wall / metabolism
Chemical Fractionation
Membrane Glycoproteins / metabolism
Porosity
Saccharomyces cerevisiae / growth & development,  metabolism*,  ultrastructure
Staining and Labeling
Chemical
Reg. No./Substance:
0/Benzenesulfonates; 0/Membrane Glycoproteins; 0/mannoproteins; 4404-43-7/C.I. Fluorescent Brightening Agent 28

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