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Cyclic stretch influenced expression of membrane connexin 43 in human periodontal ligament cell.
MedLine Citation:
PMID:  22871357     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVE: Periodontal ligament (PDL) cells play an important role in preserving periodontal homeostasis and periodontal remodelling in response to mechanical stimulations. Gap junction intercellular communication (GJIC) is essential for homeostasis and many other biological processes of multicellular organisms. While the role of GJIC in mechanotransduction of PDL cells remains largely unknown. In the present study, we examined the influence of cyclic stretch on the expression of membrane gap junction protein connexin 43 (Cx43) in cultured human PDL cells. DESIGN: Cultured human PDL cells were exposed to 1%, 10% and 20% stretch strains for 0.5h, 1h and 24h. Then the membrane Cx43 protein expression was measured by flow cytometry and the Cx43 mRNA level was measured by real-time polymerase chain reaction. RESULTS: Half hour and 1h cyclic stretches with strains up to 20% did not change the expression of membrane Cx43 protein, while 24h cyclic stretches with 10% and 20% strains down-regulated the expression of membrane Cx43 protein in a strain magnitude-dependent manner. Furthermore, cyclic stretch also changed the Cx43 mRNA level and induced realignment in cells. CONCLUSION: The present research provide the first evidence that cyclic stretch influenced the membrane Cx43 protein expression in cultured human PDL cells.
Authors:
Chun Xu; Zhen Fan; Wenting Shan; Yi Hao; Jiayin Ma; Qingfeng Huang; Fuqiang Zhang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-4
Journal Detail:
Title:  Archives of oral biology     Volume:  -     ISSN:  1879-1506     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Prosthodontics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China.
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