Document Detail

Cyclic regulation of B220 antigen expression in immature B cell lines.
MedLine Citation:
PMID:  7512534     Owner:  NLM     Status:  MEDLINE    
Three independent immature B cell lines transformed with temperature-sensitive mutants of Abelson murine leukaemia virus (A-MuLV) persistently expressed B220 antigen detected by both of the anti-B220 antibodies RA3-6B2 and 14.8 during culture at the permissive temperature (35 degrees C). RA3-6B2 recognizes the B220 eptiope and that 14.8 is a CD45RA antibody. However, when the culture temperature was shifted up to the non-permissive temperature (39 degrees C) and the culture was continued for 2-3 weeks, a population of the cells (RA3-6B2-14.8+ cells) that lost RA3-6B2 antigen appeared in all three cell lines. From these populations, RA3-6B2-14.8+ subclones were independently isolated by limiting dilution and the phenotype was stable during culture at the permissive temperature. When the culture temperature of the RA3-6B2-14.8+ subclones was shifted up to the non-permissive temperature and the culture was continued for 2-3 weeks, RA3-6B2 antigen was re-expressed in a subpopulation of the RA3-6B2-14.8+ cells. These results demonstrated cyclic regulation of B220 antigen expression during proliferation and/or differentiation of immature B cell lines.
Y Oka; Y Shimizu; S Tsukada; H Sugiyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology and cell biology     Volume:  72     ISSN:  0818-9641     ISO Abbreviation:  Immunol. Cell Biol.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-05-17     Completed Date:  1994-05-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8706300     Medline TA:  Immunol Cell Biol     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  75-8     Citation Subset:  IM    
Department of Medicine III, Osaka University Medical School, Japan.
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MeSH Terms
Antibodies, Monoclonal / immunology
Antigens, CD45
Antigens, Surface / biosynthesis*
B-Lymphocytes / immunology*
Cell Cycle
Cell Differentiation
Cell Division
Cell Line
Cell Transformation, Viral
Cells, Cultured
Flow Cytometry
Leukemia Virus, Murine
Mice, Inbred BALB C
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Surface; EC, CD45

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