Document Detail

Cyclic pressure affects osteoblast functions pertinent to osteogenesis.
MedLine Citation:
PMID:  12918906     Owner:  NLM     Status:  MEDLINE    
In an attempt to elucidate the cellular/molecular correlations between mechanical stimuli and new bone formation, the present in vitro study used a custom-made laboratory setup and examined the effects of cyclic pressure on select functions of osteoblasts pertinent to osteogenesis. The results demonstrated that, compared to controls (no pressure), mRNA expression for type-I collagen (the main constituent of the organic phase of bone) was enhanced when osteoblasts were exposed to cyclic pressure (10-40 kPa at 1.0 Hz) for 1 h daily for up to 19 consecutive days. In addition, compared to controls, both deposition of collagen and accumulation of calcium (one of the major components of the inorganic phase of bone) increased significantly (p<0.05) following exposure of osteoblast cultures to cyclic pressure for 19 days. Since the amounts of total DNA in controls and in osteoblast cultures exposed to cyclic pressure were similar at all time points tested, it was concluded that increased collagen and calcium concentrations in cultures resulted from enhanced osteoblast function (and not from increased number of cells); the presence of increased amounts of collagen affected the subsequent increased accumulation of calcium. These results provide evidence that daily exposure to cyclic pressure for various time periods (up to 19 days) affect osteoblast functions pertinent to bone formation.
Jiro Nagatomi; Bernard P Arulanandam; Dennis W Metzger; Alain Meunier; Rena Bizios
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Annals of biomedical engineering     Volume:  31     ISSN:  0090-6964     ISO Abbreviation:  Ann Biomed Eng     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-08-15     Completed Date:  2004-04-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0361512     Medline TA:  Ann Biomed Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  917-23     Citation Subset:  IM    
Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180-3590, USA.
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MeSH Terms
Animals, Newborn
Calcium / metabolism*
Cell Culture Techniques / instrumentation,  methods
Cells, Cultured
Collagen / metabolism*
Collagen Type I / metabolism
Equipment Design
Gene Expression Regulation / physiology
Mechanotransduction, Cellular / physiology*
Osteoblasts / physiology*
Osteocalcin / metabolism*
Osteogenesis / physiology*
Physical Stimulation / instrumentation,  methods*
RNA, Messenger / metabolism
Rats, Sprague-Dawley
Transforming Growth Factor beta / metabolism*
Transforming Growth Factor beta1
Grant Support
Reg. No./Substance:
0/Collagen Type I; 0/RNA, Messenger; 0/Tgfb1 protein, rat; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 104982-03-8/Osteocalcin; 7440-70-2/Calcium; 9007-34-5/Collagen

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