Document Detail

Cyclic olefin polymers: innovative materials for high-density multiwell plates.
MedLine Citation:
PMID:  18537466     Owner:  NLM     Status:  MEDLINE    
Extension of ultra-high-throughput experiment (UHTE) approaches to new assay methodologies is often limited by compromised data quality when samples are miniaturized. Overcoming this challenge requires attending to all components of an automated laboratory system contributing to assay variability. A key but often neglected source is the high-density multiwell platform or microtiter plate. Materials from which plates are fabricated may degrade or otherwise compromise an assay through a variety of sources, including structural weakness, distortion of optical signals, and chemical contamination. Cyclic olefin polymer (COP) resins (CAS Registry Number 26007-43-2, inclusive of polymers and copolymers, sometimes referred to as cyclo-olefin polymers or copolymers) are receiving attention for their structural strength, optical clarity, and biocompatibility. The physical and chemical properties of COP are reviewed for their ramifications on the performance of high-density multiwell plates. Cells known to be difficult to culture in standard plasticware thrive in miniaturized COP wells. In addition, cell-based assays whose data deteriorated when miniaturized in standard plastic reveal a robust recovery of data quality when miniaturized in COP. It is hoped that the material qualities and advantages of COP become better appreciated among the screening and biological communities.
Walter D Niles; Peter J Coassin
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Assay and drug development technologies     Volume:  6     ISSN:  1540-658X     ISO Abbreviation:  Assay Drug Dev Technol     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-09-17     Completed Date:  2008-12-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101151468     Medline TA:  Assay Drug Dev Technol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  577-90     Citation Subset:  IM    
Niles Research Corporation, 6519 Caminito Catalan, La Jolla, CA 92037, USA.
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MeSH Terms
Alkenes / chemistry*,  pharmacology
Cells, Cultured
Drug Evaluation, Preclinical / instrumentation*
Oxygen / chemistry
Polystyrenes / chemistry,  pharmacology
Quality Control
Tensile Strength
Reg. No./Substance:
0/Alkenes; 0/Polymers; 0/Polystyrenes; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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