| Cyclic olefin polymers: innovative materials for high-density multiwell plates. | |
| | |
MedLine Citation:
|
PMID: 18537466 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Extension of ultra-high-throughput experiment (UHTE) approaches to new assay methodologies is often limited by compromised data quality when samples are miniaturized. Overcoming this challenge requires attending to all components of an automated laboratory system contributing to assay variability. A key but often neglected source is the high-density multiwell platform or microtiter plate. Materials from which plates are fabricated may degrade or otherwise compromise an assay through a variety of sources, including structural weakness, distortion of optical signals, and chemical contamination. Cyclic olefin polymer (COP) resins (CAS Registry Number 26007-43-2, inclusive of polymers and copolymers, sometimes referred to as cyclo-olefin polymers or copolymers) are receiving attention for their structural strength, optical clarity, and biocompatibility. The physical and chemical properties of COP are reviewed for their ramifications on the performance of high-density multiwell plates. Cells known to be difficult to culture in standard plasticware thrive in miniaturized COP wells. In addition, cell-based assays whose data deteriorated when miniaturized in standard plastic reveal a robust recovery of data quality when miniaturized in COP. It is hoped that the material qualities and advantages of COP become better appreciated among the screening and biological communities. |
| | |
Authors:
|
Walter D Niles; Peter J Coassin |
Related Documents
:
|
19618686 - Study on migration of melamine from food packaging materials on markets. 16438306 - Analysis of acrylamide in food: dedicated to professor dr. werner baltes. 22610436 - Thermal inactivation of infectious hepatitis e virus in experimentally contaminated food. 15712526 - Migration of bisphenol a from can coatings--effects of damage, storage conditions and h... 18477176 - Lay food and health worker involvement in community nutrition and dietetics in england:... 17161186 - Pediatric gastroesophageal varices: treatment strategy and long-term results. |
Publication Detail:
|
Type: Journal Article; Review |
Journal Detail:
|
Title: Assay and drug development technologies Volume: 6 ISSN: 1540-658X ISO Abbreviation: Assay Drug Dev Technol Publication Date: 2008 Aug |
Date Detail:
|
Created Date: 2008-09-17 Completed Date: 2008-12-05 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101151468 Medline TA: Assay Drug Dev Technol Country: United States |
Other Details:
|
Languages: eng Pagination: 577-90 Citation Subset: IM |
Affiliation:
|
Niles Research Corporation, 6519 Caminito Catalan, La Jolla, CA 92037, USA. lane@laneniles.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alkenes
/
chemistry*,
pharmacology Biotechnology Cells, Cultured Drug Evaluation, Preclinical / instrumentation* Nanotechnology Oxygen / chemistry Polymers Polystyrenes / chemistry, pharmacology Quality Control Tensile Strength Viscosity |
| Chemical | |
Reg. No./Substance:
|
0/Alkenes; 0/Polymers; 0/Polystyrenes; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Phospholipidosis assay in HepG2 cells and rat or rhesus hepatocytes using phospholipid probe NBD-PE.
Next Document: Effects of verum acupuncture compared to placebo acupuncture on quantitative EEG and heart rate vari...