Document Detail

Cyclic-AMP level and eicosanoid release from alveolar macrophages are differentially affected by high and low dose of platelet activating factor.
MedLine Citation:
PMID:  8117329     Owner:  NLM     Status:  MEDLINE    
Antigen challenged alveolar macrophages (ac-AM) showed much higher basal prostaglandin E2 (PGE2) release (4,4-fold) and cAMP content (2,4-fold) than naive alveolar macrophages (AM). In naive AM 1 fM platelet activating factor (PAF) enhanced PGE2 release from 115 to 157 ng/5 x 10(6) cells but was inactive at 1 nM or 1 microM. In ac-AC 1 fM PAF enhanced PGE2 release from 510 to 670 ng/5 x 10(6) cells and inhibited leukotriene B4 (LTB4) release (from 6.0 to 4.8 ng/5 x 10(6) cells). At a 10(6)-fold higher concentration PAF inhibited PGE2 release (from 510 to 400 ng/5 x 10(6) cells) and stimulated LTB4 release (from 6.0 to 8.2 ng/5 x 10(6) cells). PAF-induced increase or decrease in PGE2 release was paralleled by changes in cellular cAMP (+35 and -17%, respectively). The specific PAF-antagonist BN 52021 completely reversed all PAF-induced effects while indomethacin inhibited only PAF-induced increase in PGE2 release and cAMP leaving LTB4 release unaffected. Similarly, the lipoxygenase inhibitor AA-861 inhibited PAF-induced rise in LTB4 release leaving the enhancement in PGE2 release and cAMP content unaffected. Present data show that PAF dose-dependently affects eicosanoid production and cAMP level in alveolar macrophages.
F D Beusenberg; I L Bonta; J G van Amsterdam
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical pharmacology     Volume:  47     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-03-25     Completed Date:  1994-03-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  588-90     Citation Subset:  IM    
Department of Pharmacology, Erasmus University Rotterdam, The Netherlands.
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MeSH Terms
Cyclic AMP / metabolism*
Dinoprostone / metabolism*
Dose-Response Relationship, Drug
Guinea Pigs
Leukotriene B4 / metabolism*
Macrophages, Alveolar / drug effects*,  metabolism
Platelet Activating Factor / pharmacology*
Reg. No./Substance:
0/Platelet Activating Factor; 363-24-6/Dinoprostone; 60-92-4/Cyclic AMP; 71160-24-2/Leukotriene B4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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