Document Detail

Cyclic AMP-induced expression of steroidogenic acute regulatory protein is dependent upon phosphoprotein phosphatase activities.
MedLine Citation:
PMID:  10750024     Owner:  NLM     Status:  MEDLINE    
In addition to the well-documented role of protein kinases in the regulation of steroid production, phosphoprotein phosphatase (PP) activity is required for steroidogenesis. In the present study, we have used the mouse Y1 adrenocortical cell line to identify the site of action of PPs on steroid production by measuring the effects of PP inhibition on the expression of the steroidogenic acute regulatory (StAR) protein and on steroid production. Forskolin-induced activation of cyclic AMP-dependent protein kinase (PKA) enhanced steroidogenesis and this was accompanied by an increased expression of StAR protein. Both steroidogenesis and StAR protein expression were inhibited by two structurally dissimilar inhibitors of PP1 and PP2A activities, okadaic acid and calyculin A. These results suggest that inhibition of PP1 and PP2A inhibits steroid production by preventing the expression of the StAR protein, implicating PP1/2A dephosphorylation reactions as important regulators of stimulus-dependent StAR protein expression, and thus of steroidogenesis.
P M Jones; S B Sayed; S J Persaud; C J Burns; S Gyles; B J Whitehouse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of molecular endocrinology     Volume:  24     ISSN:  0952-5041     ISO Abbreviation:  J. Mol. Endocrinol.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-05-08     Completed Date:  2000-05-08     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8902617     Medline TA:  J Mol Endocrinol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  233-9     Citation Subset:  IM    
Endocrinology and Reproduction Research Group, School of Biomedical Sciences, King's College London, New Hunt's House, Guy's Campus, London SE1 1UL, UK.
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MeSH Terms
Adrenal Cortex / metabolism*
Cell Line
Cyclic AMP / metabolism*
Cyclic AMP-Dependent Protein Kinases / metabolism*
Enzyme Inhibitors / pharmacology
Forskolin / pharmacology
Hydroxycholesterols / pharmacology
Isoenzymes / metabolism
Okadaic Acid / pharmacology
Oxazoles / pharmacology
Phosphoproteins / biosynthesis*,  genetics
Pregnenolone / pharmacology
Progesterone / metabolism
Protein Tyrosine Phosphatases / metabolism*
Steroids / biosynthesis*
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Hydroxycholesterols; 0/Isoenzymes; 0/Oxazoles; 0/Phosphoproteins; 0/Steroids; 0/steroidogenic acute regulatory protein; 101932-71-2/calyculin A; 145-13-1/Pregnenolone; 17711-16-9/22-hydroxycholesterol; 57-83-0/Progesterone; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; 78111-17-8/Okadaic Acid; EC AMP-Dependent Protein Kinases; EC Tyrosine Phosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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